Insights into the mechanism of shortened developmental duration and accelerated weight gain associated with Wolbachia infection in Hylyphantes graminicola.
Insights into the mechanism of shortened developmental duration and accelerated weight gain associated with Wolbachia infection in Hylyphantes graminicola.
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深入了解 Hylyphantes graminicola 中与沃尔巴克氏体感染相关的缩短发育持续时间和加速体重增加的机制。
DOI：
10.1111/1749-4877.12550
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发表时间：
2021-04
影响因子：
3.3
通讯作者：
Yueli Yun
中科院分区：
文献类型：
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作者：
Meng He;Yinjie Cui;Y U Peng;Chunfen Li;Jie Liu;Yueli Yun
已知沃尔巴克氏体感染会影响宿主的生殖和发育。然而，到目前为止，尚未报道与沃尔巴克氏体对宿主发育的影响有关的潜在机制。比较了Wolbachia阳性（W+）和Wolbachia阴性（W-）草间屋蛛（Hylyphantes graminicola）不同龄期的发育历期和体重，并采用逆转录qPCR（RT-qPCR）技术检测了6个胰岛素相关基因和3个蜕皮激素相关基因的相对表达水平。结果表明，与W-蜘蛛相比，W+蜘蛛的发育历期显著缩短.此外，在3龄和4龄时，W+蜘蛛的体重显著高于W-蜘蛛，但成熟后的体重没有显著差异。我们还发现，与W-蜘蛛相比，W+蜘蛛中胰岛素样生长因子-2 mRNA结合蛋白-1、胰岛素降解酶和蜕皮激素诱导蛋白-1基因的表达水平显著下调，而W+蜘蛛中胰岛素样生长因子结合蛋白-1、胰岛素样肽受体、胰岛素受体底物2-B、胰岛素样蜕皮激素诱导蛋白-2、蜕皮激素受体基因在W+蜘蛛中表达显著上调。我们的研究结果表明，沃尔巴克氏体可能会影响主机的发展，通过影响胰岛素和蜕皮激素信号通路。本文受版权保护。All rights reserved.
Wolbachia infection is known to affect host reproduction and development. To date, however, the underlying mechanism related to the effects of Wolbachia on host development has not yet been reported. Here, we compared the developmental duration and body weight in different instars of Wolbachia-positive (W+ ) and Wolbachia-negative (W- ) spiders (Hylyphantes graminicola) and detected the relative expression levels of six insulin-related genes and three ecdysone-related genes using reverse transcription qPCR (RT-qPCR). Results showed that the developmental duration was significantly shortened in W+ spiders compared with W- spiders. Furthermore, W+ spiders were significantly heavier than W- spiders at the 3rd and 4th instars, although no significant differences in body weight were observed after maturity. We also found that the expression levels of insulin-like growth factor-2 mRNA-binding protein-1, insulin-degrading enzyme, and ecdysone-inducible protein-1 genes were significantly down-regulated in W+ spiders compared with W- spiders, whereas the expression levels of insulin-like growth factor-binding protein-1, insulin-like peptide receptor, insulin receptor substrate 2-B, insulin-like, ecdysone-induced protein-2, and ecdysone receptor genes were significantly up-regulated in W+ spiders. Our results suggest that Wolbachia may influence host development by affecting insulin and ecdysone signaling pathways. This article is protected by copyright. All rights reserved.