Effect of vulculic acid produced by Nimbya alternantherae on the photosynthetic apparatus of Alternanthera philoxeroides
Effect of vulculic acid produced by Nimbya alternantherae on the photosynthetic apparatus of Alternanthera philoxeroides
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DOI：
10.1016/j.plaphy.2013.01.013
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发表时间：
2013-04-01
影响因子：
6.5
通讯作者：
Strasser, Reto Joerg
中科院分区：
文献类型：
--
作者：
Xiang, Meimei;Chen, Shiguo;Strasser, Reto Joerg
通过光化学活性、类囊体膜蛋白质的SOS-PAGE电泳、叶绿素a荧光快速瞬变测定和JIP检验，研究了互隔莲子草（Alternanthera alternantherae）产生的毒素Vulculic acid对空心莲子草光合机构的影响。结果表明，经戊二酸处理后，光系统II的电子传递速率、非环磷酸化活性、叶绿体ATPase和Rubisco活性均显著降低。Vulcuic acid影响O-J-I-P荧光诱导动力学，表现为参数F-V/F-O、V-K和V-J增加，F-O、F-M、PIABS、（phi Po）、（psi Eo）、（phi Eo）、（phi Ro）、delta（Ro）和PI总减少。此外，它显着降低了主要的光系统I（PSI）和PSII蛋白的量。因此，挥发酸是一种具有多个作用位点的光合作用抑制剂。主要靶点是PSII供体侧的捕光复合物（LHC）和释氧复合物（OEC）。Vulculic acid通过消化主要的PSI和PSII蛋白质阻断Q（A）之外和PSI受体侧的电子传递。(C)2013年Elsevier Masson SAS。All rights reserved.
The effect of the toxin vulculic acid produced by Nimbya alternantherae, on the photosynthetic apparatus of Alternanthera philoxeroides, was investigated via the photochemical activity and SOS-PAGE of protein on thylakoid membranes, fast chlorophyll a fluorescence transient measurements and the JIP-test. The electron transport rate of photosystem II (PSII), non-cyclic photophosphorylation activity, as well as the activity of chloroplast ATPase and Rubisco reduced significantly after vulculic acid treatment. Vulculic acid affected the O-J-I-P fluorescence induction kinetics, showing an increase of the parameters F-V/F-O, V-K and V-J and a decrease of F-O, F-M, PIABS, (phi Po), (psi Eo), (phi Eo), (phi Ro), delta(Ro) and PItotal. In addition, it significantly decreased the amounts of major photosystem I (PSI) and PSII proteins. It is concluded that vulculic acid is a photosynthetic inhibitor with multiple action sites. The main targets are the light harvesting complex (LHC) and the oxygen evolving complex (OEC) on the PSII donor side. Vulculic acid blocks electron transport beyond Q(A) and on the PSI acceptor side by digesting major PSI and PSII proteins. (C) 2013 Elsevier Masson SAS. All rights reserved.