Ultrasound-Induced Cascade Amplification in a Mechanoluminescent Nanotransducer for Enhanced Sono-Optogenetic Deep Brain Stimulation.
Ultrasound-Induced Cascade Amplification in a Mechanoluminescent Nanotransducer for Enhanced Sono-Optogenetic Deep Brain Stimulation.
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DOI：
10.1021/acsnano.3c06577
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发表时间：
2023-12
期刊：
影响因子：
17.1
通讯作者：
Wenliang Wang;Kai Wing Kevin Tang;Ilya Pyatnitskiy;Xiangping Liu;Xi Shi;David Huo;Jinmo Jeong;Thomas Wynn;Arjun Sangani;Andrew Baker;Ju-Chun Hsieh;Anakaren Romero Lozano;Brinkley Artman;L. Fenno;Vivek P Buch;Huiliang Wang
中科院分区：
文献类型：
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作者：
Wenliang Wang;Kai Wing Kevin Tang;Ilya Pyatnitskiy;Xiangping Liu;Xi Shi;David Huo;Jinmo Jeong;Thomas Wynn;Arjun Sangani;Andrew Baker;Ju-Chun Hsieh;Anakaren Romero Lozano;Brinkley Artman;L. Fenno;Vivek P Buch;Huiliang Wang
大脑深部的远程和基因靶向神经调节对于了解和治疗神经疾病非常重要。超声波触发的机械发光技术为实现远程和基因靶向的大脑调制提供了一种很有前途的方法。然而，到目前为止，由于声化学反应效率低和光子产量有限的挑战，它的应用仅限于大脑浅层。在这里，我们报告了一个级联的机械发光纳米换能器，以实现有效的光发射的超声波刺激。结果，在超声刺激下产生蓝光，反应潜伏期为亚秒。利用聚焦超声在脑组织中的高能量转移效率和这些机械发光纳米换能器对超声波的高敏感性，我们能够显示在脑内注射后，运动皮质浅层和腹侧被盖深部的ChR2表达神经元的高效光子传递和激活。我们的脂质体纳米换能器能够通过灵活的机械发光声光遗传系统实现微创脑深部刺激，以控制动物的行为。
Remote and genetically targeted neuromodulation in the deep brain is important for understanding and treatment of neurological diseases. Ultrasound-triggered mechanoluminescent technology offers a promising approach for achieving remote and genetically targeted brain modulation. However, its application has thus far been limited to shallow brain depths due to challenges related to low sonochemical reaction efficiency and restricted photon yields. Here we report a cascaded mechanoluminescent nanotransducer to achieve efficient light emission upon ultrasound stimulation. As a result, blue light was generated under ultrasound stimulation with a subsecond response latency. Leveraging the high energy transfer efficiency of focused ultrasound in brain tissue and the high sensitivity to ultrasound of these mechanoluminescent nanotransducers, we are able to show efficient photon delivery and activation of ChR2-expressing neurons in both the superficial motor cortex and deep ventral tegmental area after intracranial injection. Our liposome nanotransducers enable minimally invasive deep brain stimulation for behavioral control in animals via a flexible, mechanoluminescent sono-optogenetic system.