Production of antibodies by single DNA immunization: Comparison of various immunization routes
Production of antibodies by single DNA immunization: Comparison of various immunization routes
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DOI：
10.1089/153685902760213903
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发表时间：
2002-08-01
期刊：
影响因子：
--
通讯作者：
Chawansuntati, K
中科院分区：
文献类型：
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作者：
Kasinrerk, W;Moonsom, S;Chawansuntati, K
DNA免疫是近年来发展起来的一种疫苗接种方法，它能在多种宿主中诱导体液和细胞免疫应答。不同的免疫途径诱导不同程度的免疫应答。在本报告中，我们证明，多次肌肉注射免疫的质粒DNA编码的各种白细胞表面分子诱导特异性抗体反应。相反，单次肌肉内免疫不能诱导抗体产生。为了研究质粒DNA单次免疫后抗体应答的诱导，同时用编码CD 147膜蛋白的质粒DNA的相同制备物对小鼠进行单剂量肌内、腹膜内、静脉内和脾内免疫。我们观察到，只有脾内途径诱导特异性抗体产生。用编码CD 54分子的质粒DNA证实脾内免疫诱导抗体。通过这种单剂量DNA脾内免疫，产生的抗体可以在小鼠中检测到长达6个月。这些结果表明，注射的DNA在注射后几个月内在脾脏中表达相关蛋白抗原。我们的研究结果表明，直接免疫的抗原编码DNA到脾脏是一个更有效的方法诱导抗体的产生。这一发现可能支持未来的DNA疫苗接种策略，特别是促进脾细胞的质粒摄取的调查。脾内免疫也有助于了解DNA疫苗免疫应答的早期事件，并可作为诱导免疫应答的有效途径。
DNA immunization is a recent vaccination method that induces humoral and cellular immune responses in a range of hosts. Different immunization routes induce a different degree of the immune response. In the present report, we demonstrate that multiple intramuscular immunizations of plasmid DNA encoding various leukocyte surface molecules induced a specific antibody response. In contrast, a single intramuscular immunization could not induce antibody production. To study the induction of antibody response after a single immunization of plasmid DNA, mice were single-dose intramuscularly, intraperitoneally, intravenously and intrasplenically immunized, simultaneously, with the same preparation of plasmid DNA encoding CD147 membrane protein. We observed that only the intrasplenic route induced specific antibody production. The induction of antibody by intrasplenic immunization was confirmed by using plasmid DNA encoding CD54 molecule. By this single-dose DNA intrasplenic immunization, the generated antibodies could be detected in mice up to 6 months. These results suggest that the injected DNA is expressing the relevant protein antigen in the spleen for several months after injection. Our results demonstrate that direct immunization of antigen-encoding DNA into the spleen is a more effective method for induction of antibody production. This finding may support future investigations of DNA vaccination strategies that specifically promote the uptake of plasmid by splenocytes. Intrasplenic immunization may also be helpful in the understanding of the early events of the immune response to DNA vaccine and be useful as an effective route for the induction of immune responses.