Cloning of a human kidney cDNA with similarity to the sodium-glucose cotransporter.
Cloning of a human kidney cDNA with similarity to the sodium-glucose cotransporter.
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DOI：
10.1152/ajprenal.1992.263.3.f459
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发表时间：
1992-09
期刊：
影响因子：
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通讯作者：
Rebecca G. Wells;A. Pajor;Y. Kanai;E. Turk;E. M. Wright;M. Hediger
中科院分区：
文献类型：
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作者：
Rebecca G. Wells;A. Pajor;Y. Kanai;E. Turk;E. M. Wright;M. Hediger
我们用人肠道Na(+)-葡萄糖共转运蛋白SGLT1进行低严格性筛选，从人肾中分离出2,271个核苷酸的cDNAHu14。该克隆编码一个672个残基的蛋白质，在氨基酸水平上与SGLT1有59%的同源性，并具有类似的预测跨膜区域的数量和排列。它还与其他Na(+)偶联转运蛋白有显著的序列同源性。Northern印迹分析表明，Hu14在肾脏中有较强的表达，但与SGLT1不同，在肠道中没有显著表达。我们一直无法证明任何一种底物(包括氨基酸、糖、核苷和维生素)确实可以转运到Hu14 cRNA注射的非洲爪哇卵母细胞中，尽管序列保守使Hu14很可能代表Na+共转运体家族的另一个成员，可能是第二个Na(+)-葡萄糖共转运体。
We have used low-stringency screening with the human intestinal Na(+)-glucose cotransporter SGLT1 to isolate a 2,271-nucleotide cDNA (Hu14) from human kidney. This clone, which encodes a 672-residue protein, is 59% identical at the amino acid level to SGLT1 and has a similar number and arrangement of predicted membrane-spanning regions. It also shares significant sequence identity with other Na(+)-coupled transporters. Northern blot analysis suggests strong expression of Hu14 in kidney, but, unlike SGLT1, no significant expression in intestine. We have been unable to demonstrate definitive transport of any of a number of substrates (including amino acids, sugars, nucleosides, and vitamins) into Hu14 cRNA-injected Xenopus oocytes, although sequence conservation makes it likely that Hu14 represents another member of the Na+ cotransporter family, possibly a second Na(+)-glucose cotransporter.