Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturing
Generation of reference cell lines, media, and a process platform for CHO cell biomanufacturing
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生成用于 CHO 细胞生物制造的参考细胞系、培养基和工艺平台
DOI：
10.1002/bit.28290
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发表时间：
2022
影响因子：
3.8
通讯作者：
Synoground, Benjamin F.
中科院分区：
文献类型：
--
作者：
Cordova, Lauren T.;Dahodwala, Hussain;Elliott, Kathryn S.;Baik, Jongyoun;Odenewelder, Daniel C.;Nmagu, Douglas;Skelton, Bradley A.;Uy, Lisa;Klaubert, Stephanie R.;Synoground, Benjamin F.
由于中国仓鼠卵巢 (CHO) 细胞在治疗性蛋白质和抗体生物制造方面具有有利的特性，公司可以生产具有理想表型的专有细胞。一个关键属性是能够在化学成分确定的培养基中稳定表达每升多克的滴度。细胞、媒体和饲料的多样性限制了社区转化知识的努力。此外，由于公众可获得性以及生成此类细胞所需的时间和知识有限，学术和非营利研究人员通常无法研究“工业相关”CHO 细胞。为了解决这些问题，一个大学工业联盟（先进哺乳动物生物制造创新中心，AMBIC）从不同谱系获得了两种表达单克隆抗体的 CHO“参考细胞系”。这些参考细胞系具有相关的生产滴度、经多个实验室确认的关键性能结果以及详细的技术转让协议。在商业培养基中，效价可达到 2 g/L 以上。提供了来自摇瓶和按比例缩小的生物反应器的分批补料培养数据。将生产力作为主要属性，两个学术站点在每个站点上都具有严格的可重复性。此外，还开发了化学成分确定的培养基配方并与商业培养基同时进行评估。这项工作的目标是提供一个通用的、工业相关的 CHO 培养平台，以加速生物制造创新。
Due to the favorable attributes of Chinese hamster ovary (CHO) cells for therapeutic proteins and antibodies biomanufacturing, companies generate proprietary cells with desirable phenotypes. One key attribute is the ability to stably express multi‐gram per liter titers in chemically defined media. Cell, media, and feed diversity has limited community efforts to translate knowledge. Moreover, academic, and nonprofit researchers generally cannot study “industrially relevant” CHO cells due to limited public availability, and the time and knowledge required to generate such cells. To address these issues, a university‐industrial consortium (Advanced Mammalian Biomanufacturing Innovation Center, AMBIC) has acquired two CHO “reference cell lines” from different lineages that express monoclonal antibodies. These reference cell lines have relevant production titers, key performance outcomes confirmed by multiple laboratories, and a detailed technology transfer protocol. In commercial media, titers over 2 g/L are reached. Fed‐batch cultivation data from shake flask and scaled‐down bioreactors is presented. Using productivity as the primary attribute, two academic sites aligned with tight reproducibility at each site. Further, a chemically defined media formulation was developed and evaluated in parallel to the commercial media. The goal of this work is to provide a universal, industrially relevant CHO culture platform to accelerate biomanufacturing innovation.
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影响因子：
3.5
作者：
Chitwood DG;Wang Q;Elliott K;Bullock A;Jordana D;Li Z;Wu C;Harcum SW;Saski CA
通讯作者：
Saski CA
Healthy People 2010 数据抑制标准。
DOI：
--
发表时间：
2002
期刊：
Healthy People 2010 statistical notes : from the Centers for Disease Control and Prevention/National Center for Health Statistics
影响因子：
--
作者：
Richard J. T. Klein;S. Proctor;Manon A Boudreault;K. Turczyn
通讯作者：
K. Turczyn
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影响因子：
3.8
作者：
Hamaker, Nathaniel K.;Min, Lie;Lee, Kelvin H.
通讯作者：
Lee, Kelvin H.
影响因子：
3.8
作者：
Hilliard, William;Lee, Kelvin H.
通讯作者：
Lee, Kelvin H.
影响因子：
4.7
作者：
Synoground，Benjamin F.;McGraw，Claire E.;Sandoval，Nicholas R.
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