Evaluation of chlorophyll content and fluorescence parameters as indicators of drought tolerance in barley.
Evaluation of chlorophyll content and fluorescence parameters as indicators of drought tolerance in barley.
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DOI：
10.1016/s1671-2927(06)60120-x
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发表时间：
2006-01-01
影响因子：
--
通讯作者：
Guo, P. G.
中科院分区：
文献类型：
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作者：
Li RongHua, Li RongHua;Guo PeiGuo, Guo PeiGuo;Guo, P. G.
干旱是一种严重影响全球粮食生产的主要非生物胁迫。与耐旱性相关的农艺和生理性状是选择耐旱基因型的合适指标，以减少育种计划中水分亏缺对作物产量的影响。本研究旨在通过分析大麦（Hordeum vulgare）的光合性状来确定与耐旱性相关的指标。这些性状包括叶绿素含量、初始荧光(Fo)、光系统II的光化学最大初级产率(Fv/Fo)和光系统II的最大量子产率(Fv/Fm)。利用耐旱性的四种基因型（Tadmor、Arta、Morocco9-75 和 WI2291）来研究这些性状与耐旱性之间的相关性。结果表明，4个基因型在不同程度的花后干旱胁迫下，所有这些性状均受到负面影响，但耐旱基因型的下降幅度远小于干旱敏感基因型。研究结果进一步表明，干旱敏感基因型的光合机构组件会受到显着损害，而耐旱基因型受到的影响相对较小。另一方面，在干旱胁迫下，耐旱基因型的叶绿素含量、Fo、Fv/Fo和Fv/Fm值显着高于干旱敏感基因型。结论：叶绿素含量、Fo、Fv/Fo、Fv/Fm可作为筛选大麦种质耐旱性的可靠指标。
Drought is a major abiotic stress that severely affects food production worldwide. Agronomic and physiological traits associated with drought tolerance are suitable indicators for selection of drought-tolerant genotypes to reduce the impact of water deficit on crop yield in breeding programmes. This study was conducted to identify indicators related to drought tolerance through analysis of photosynthetic traits in barley (Hordeum vulgare). These traits included chlorophyll content, initial fluorescence (Fo), maximum primary yield of photochemistry of photosystem II (Fv/Fo) and maximum quantum yield of photosystem II (Fv/Fm). Four genotypes (Tadmor, Arta, Morocco9-75 and WI2291) variable in drought tolerance were used to investigate the correlation between these traits and drought tolerance. The results reflected that all of these traits were affected negatively in the four genotypes at different levels of postanthesis drought stress, but the decrease in drought-tolerant genotypes was much less than that of drought-sensitive genotypes. The results further revealed that the components of the photosynthetic apparatus could be damaged significantly in drought-sensitive genotypes, while drought-tolerant genotypes were relatively less affected. On the other hand, the values of chlorophyll content, Fo, Fv/Fo and Fv/Fm in drought-tolerant genotypes were significantly higher than those in drought-sensitive genotypes under drought stress. It is concluded that chlorophyll content, Fo, Fv/Fo and Fv/Fm could be considered as reliable indicators in screening barley germplasm for drought tolerance.