Enhanced nitrobenzene biotransformation by graphene‐anaerobic sludge composite
Enhanced nitrobenzene biotransformation by graphene‐anaerobic sludge composite
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DOI：
10.1002/jctb.4182
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发表时间：
2014-05
影响因子：
3.4
通讯作者：
Jing Wang;Di Wang;Guangfei Liu;Ruofei Jin;Hong Lu
中科院分区：
文献类型：
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作者：
Jing Wang;Di Wang;Guangfei Liu;Ruofei Jin;Hong Lu
传统的厌氧生物工艺无法实现硝基苯的高效生物转化。因此，石墨烯-厌氧污泥复合材料作为一种新型生物催化剂被提出用于硝基苯的生物转化。结果将还原氧化石墨烯/厌氧污泥（RGO/AS）复合材料添加到氧化石墨烯（GO）体系中培养24 h后，其沉降性能良好。还原氧化石墨烯的存在增强了AS对硝基苯的生物转化，当初始氧化石墨烯（3-5µm）与污泥比为0.075 （w/w）时，硝基苯的去除率最高。RGO/AS系统的脱氢酶活性比AS系统增加了约2倍，RGO/AS系统的上清中出现了氧化还原活性物质。在葡萄糖发酵过程中，RGO/AS体系中乙酸与丙酸的比例增加，并且AS和RGO/AS体系对硝基苯的生物转化与产甲烷无关，但依赖于产丙酮。此外，RGO/AS复合材料中结合的和游离的胞外聚合物（EPS）参与了硝基苯的直接生物转化，结合的EPS可能与分泌的氧化还原活性物质相互作用，通过胞外电子转移加速硝基苯的生物转化。结论RGO/AS复合材料处理硝基苯废水效果良好。©2013化工学会
BACKGROUND Traditional anaerobic bioprocesses have failed to achieve the efficient biotransformation of nitrobenzene. Thus, graphene-anaerobic sludge composite as a novel biocatalyst was proposed for the enhancement of nitrobenzene biotransformation. RESULTS Reduced graphene oxide/anaerobic sludge (RGO/AS) composite presented good settling performance when graphene oxide (GO) was added into an AS system for 24 h cultivation. The presence of RGO resulted in enhanced nitrobenzene biotransformation by AS, and the highest removal efficiency of nitrobenzene was observed with initial GO (3–5 µm) vs sludge ratio of 0.075 (w/w). Dehydrogenase activity in the RGO/AS system increased approximately 2-fold over that in the AS system and redox active species appeared in supernatant from the RGO/AS system. The ratio of acetate to propionate increased in the RGO/AS system during glucose fermentation, and nitrobenzene biotransformation by both AS and RGO/AS systems was independent of methanogenesis, but dependent on acetogenesis. Moreover, bound and free extracellular polymeric substances (EPS) from RGO/AS composite were involved in direct biotransformation of nitrobenzene, and bound EPS might interact with secreted redox active species to accelerate nitrobenzene biotransformation by extracellular electron transfer. CONCLUSION RGO/AS composite was efficient for the treatment of nitrobenzene wastewater. © 2013 Society of Chemical Industry