Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase
Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase
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DOI：
10.1046/j.1365-3024.2000.00260.x
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发表时间：
2000-01-01
影响因子：
2.2
通讯作者：
Rodrigues, MM
中科院分区：
文献类型：
--
作者：
Ribeirao, M;Pereira-Chioccola, VL;Rodrigues, MM
克氏锥虫（引起恰加斯病的寄生虫）的感染形式在其表面表达一种名为转唾液酸酶（TS）的酶。本研究旨在评估慢性感染恰加斯个体对代表 TS 催化结构域的细菌重组蛋白的自然获得性免疫反应。通过体外 T 细胞增殖以及对全寄生虫匀浆和重组蛋白的干扰素 (INF)-γ、白介素 (IL)-4 和 IL-10 产生来测量细胞免疫反应。根据 T 细胞增殖估计，28 名恰加斯病患者中 78.6% 的外周血单核细胞对重组蛋白有反应。关于细胞因子的产生，恰加斯个体的 88% 的细胞在重组蛋白的刺激下产生了 IFN-γ。相比之下，TS 产生的 IL-4 或 IL-10 最少。细胞免疫反应是特异性的，因为大多数从未接触过克氏锥虫的健康个体未能与这种重组蛋白发生反应。分别通过 ELISA 或 TS 抑制抗体的存在测定，71.4% 或 100% 恰加斯病患者的血浆中含有 Ige 抗体。我们得出的结论是，在大部分感染克氏锥虫的患者中，TS 的催化结构域被产生 IFN-γ 的 I 型细胞和抗体所识别。
Infective forms of Trypanosoma cruzi, the parasite that causes Chagas' disease, express on their surface an enzyme denominated trans-sialidase (TS). The present study was designed to evaluate the naturally acquired immune responses to a bacterial recombinant protein representing the catalytic domain of TS in chronically infected chagasic individuals. The cellular immune response was measured by in-vitro T-cell proliferation and by interferon (INF)-gamma, interleukin (IL)-4 and IL-10 production in response to a whole-parasite homogenate and the recombinant protein. The peripheral blood mononuclear cells of 78.6% of 28 chagasic patients responded to the recombinant protein as estimated by T-cell proliferation. With respect to cytokine production, 88% of the cells of the chagasic individuals produced IFN-gamma on stimulation with the recombinant protein. In contrast, IL-4 or IL-10 were minimally produced in response to TS. The cellular immune response was specific because most healthy individuals never exposed to T. cruzi failed to react with this recombinant protein. The plasma of 71.4% or 100% of chagasic patients had Ige antibodies as determined by ELISA or by the presence of TS inhibitory antibodies, respectively. We conclude that the catalytic domain of TS is recognized by IFN-gamma producing type I cells and antibodies in a large proportion of patients infected with T. cruzi.