Recurrent Laryngeal Nerve Reinnervation in Rats Posttransection: Neurotrophic Factor Expression over Time
Recurrent Laryngeal Nerve Reinnervation in Rats Posttransection: Neurotrophic Factor Expression over Time
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DOI：
10.1177/0194599819831289
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发表时间：
2019-07-01
影响因子：
3.4
通讯作者：
Pitman, Michael J.
中科院分区：
文献类型：
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作者：
Montalbano, Michael B.;Hernandez-Morato, Ignacio;Pitman, Michael J.
目的喉返神经（RLN）损伤引起声带麻痹，由于非选择性神经再生，声带功能无法恢复。本研究通过检测神经胶质源性神经营养因子（GDNF）、netrin 1和层粘连蛋白111 （LAMA1）在非聚集性喉部肌肉中的表达，研究轴突引导信号及其调节剂与再神经支配年代学的关系。这项研究首次描述了rln损伤后LAMA1的表达模式，这是一个特别有趣的目标，因为它已被证明可以将网状蛋白1介导的生长锥吸引转换为排斥。动物实验（大鼠模型）。基础科学实验室。方法64只雌性Sprague-Dawley大鼠，分别于伤后1、3、7、21、28、56 d切除右侧RLNs。从同侧环状后杓（PCA）、外侧甲状腺杓（LTA）和内侧甲状腺杓（MTA）中分离单动物信使RNA进行定量逆转录聚合酶链反应（qRT-PCR）分析。在相同的肌肉中进行LAMA1表达的免疫染色。结果PCA在3 ~ 56 DPI时LAMA1升高，LTA在7 DPI时升高，MTA在14、28 DPI时升高。这与喉神经再支配的时间顺序有关。使用新的方案，单动物肌肉qRT-PCR可能和GDNF和netrin 1的表达结果与之前的合并研究相似。结论对单个大鼠喉部肌肉进行定量定量rt - pcr检测是可行的。netrin 1和LAMA1的表达在时间上与LTA和MTA的肌肉神经支配相协调。这表明LAMA1可能影响网络蛋白1排斥轴突，延迟LTA和MTA神经再生。
Objective Recurrent laryngeal nerve (RLN) injury causes vocal fold paralysis from which functional recovery is typically absent due to nonselective reinnervation. This study investigates expression of axon guidance cues and their modulators relative to the chronology of reinnervation by examining the expression of glial-derived neurotrophic factor (GDNF), netrin 1, and laminin 111 (LAMA1) in nonpooled laryngeal muscles. This study is the first to describe the post-RLN injury expression pattern of LAMA1, a target of particular interest as it has been shown to switch netrin 1-mediated growth cone attraction to repulsion. Study Design Animal experiment (rat model). Setting Basic science laboratory. Methods The right RLNs of 64 female Sprague-Dawley rats were transected, with sacrifice at 1, 3, 7, 21, 28, and 56 days postinjury (DPI). Single-animal messenger RNA was isolated from the ipsilateral posterior cricoarytenoid (PCA), lateral thyroarytenoid (LTA), and medial thyroarytenoid (MTA) for quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. Immunostaining for LAMA1 expression was performed in the same muscles. Results LAMA1 was elevated in the PCA at 3 to 56 DPI, LTA at 7 DPI, and MTA at 14 and 28 DPI. This correlates with the chronology of laryngeal reinnervation. Using a new protocol, single-animal muscle qRT-PCR possible and expression results for GDNF and netrin 1 were similar to previous pooled investigations. Conclusion Reliable qRT-PCR is possible with single rat laryngeal muscles. The expression of netrin 1 and LAMA1 is chronologically coordinated with muscle innervation in the LTA and MTA. This suggests that LAMA1 may influence netrin 1 to repel axons and delay LTA and MTA reinnervation.