Immunogenicity of West Nile virus infectious DNA and its noninfectious derivatives.
Immunogenicity of West Nile virus infectious DNA and its noninfectious derivatives.
复制标题
西尼罗河病毒感染性 DNA 及其非感染性衍生物的免疫原性。
DOI：
10.1016/j.virol.2006.07.038
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发表时间：
2006
期刊：
影响因子：
3.7
通讯作者：
Yamshchikov,Vladimir
中科院分区：
文献类型：
--
作者：
Seregin,Alexey;Nistler,Ryan;Borisevich,Victoria;Yamshchikov,Galina;Chaporgina,Elena;Kwok,ChunWai;Yamshchikov,Vladimir
西尼罗NY99毒株的极高毒力使其在WN活疫苗开发中的适用性不确定。本研究的目的是研究携带假致死突变的非感染性病毒衍生物的免疫原性，该突变可在不影响病毒感染周期的前几个步骤的情况下阻止病毒体形成。当使用DNA免疫施用时，此类构建体启动感染循环，但不会导致病毒血症。虽然对非感染性复制能力构建体的免疫应答的幅度低于病毒或感染性DNA，但其总体质量和保护作用相似。相比之下，相似长度的非复制构建体在所用剂量范围内仅诱导轻微可检测的免疫应答。因此，衍生自感染性DNA的可复制非感染性构建体可以提供非感染性制剂的安全性与感染性疫苗的免疫应答特性的质量的有利组合。
The exceptionally high virulence of the West Nile NY99 strain makes its suitability in the development of a live WN vaccine uncertain. The aim of this study is to investigate the immunogenicity of noninfectious virus derivatives carrying pseudolethal mutations, which preclude virion formation without affecting preceding steps of the viral infectious cycle. When administered using DNA immunization, such constructs initiate an infectious cycle but cannot lead to a viremia. While the magnitude of the immune response to a noninfectious replication-competent construct was lower than that of virus or infectious DNA, its overall quality and the protective effect were similar. In contrast, a nonreplicating construct of similar length induced only a marginally detectable immune response in the dose range used. Thus, replication-competent noninfectious constructs derived from infectious DNA may offer an advantageous combination of the safety of noninfectious formulations with the quality of the immune response characteristic of infectious vaccines.