Aflatoxin B1 Electrochemical Aptasensor Based on Tetrahedral DNA Nanostructures Functionalized Three Dimensionally Ordered Macroporous MoS2-AuNPs Film
Aflatoxin B1 Electrochemical Aptasensor Based on Tetrahedral DNA Nanostructures Functionalized Three Dimensionally Ordered Macroporous MoS2-AuNPs Film
复制标题
基于四面体DNA纳米结构功能化三维有序大孔MoS2-AuNPs薄膜的黄曲霉毒素B1电化学适体传感器
DOI：
10.1021/acsami.8b01693
复制
发表时间：
2018-05-30
影响因子：
9.5
通讯作者：
Huang, He
中科院分区：
文献类型：
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作者：
Peng, Gang;Li, Xiaoyan;Huang, He
在食品安全评价中，黄曲霉毒素B1(AFB1)是一项重要指标。在这项工作中，我们开发了一种基于四面体DNA纳米结构(TDNS)固定的三维有序大孔MoS_2-AuNPs(3DOM MoS_2-AuNPs)识别界面和辣根过氧化物酶(HRP)功能化磁信号放大器的AFB1电化学适配传感器。为了大大提高核酸适体传感器的识别效率、灵敏度和稳定性，巧妙地将AFB1适配子修饰的TDNS与3DOM MoS2-AuNPs薄膜相结合，构建了传感界面。适体与AFB1反应后从电极表面释放，形成无杂交的TDN。因此，DNA辅助链(H1)/HRP功能化的AuNPs-SiO_2@Fe_3O_4纳米球的生物复合材料将由于H1和TDN的杂交而与无杂交的TDN结合。溶液中AFB1含量越高，H1/HRP-AuNPs-SiO(2)PFe(3)O(4)在3DOM MoS2-AuNPs表面的结合越多。以THI为电化学探针的HRP催化还原H_2O_2的电流响应与黄曲霉毒素B_1的浓度成正比。在优化条件下，该适配子传感器对黄曲霉毒素B受体1有较好的特异性，线性范围为0.1fg/mL~0.1ug/mL，检出限为0.01fg/mL。此外，所研制的适配传感器还用于大米和小麦粉样品中黄曲霉毒素B_1的测定，获得了与高效液相色谱-串联质谱法相一致的结果。
In food safety evaluation, aflatoxin B1 (AFB1) is an important indicator. In this work, we developed an AFB1 electrochemical aptasensor based on a tetrahedral DNA nanostructures (TDNs) immobilized three dimensionally ordered macroporous MoS2-AuNPs hybrid (3DOM MoS2-AuNPs) recognition interface and horseradish peroxidase (HRP) functionalized magnetic signal amplifier. To greatly enhance the recognition efficiency, sensitivity, and stability of the aptasensor, the AFB1 aptamer-incorporated TDNs were ingeniously combined with the 3DOM MoS2-AuNPs film for the construction of the sensing interface. The aptamers would release from the electrode surface after they reacted with AFB1, and then the hybridization-free TDNs formed. Thus, the biocomposite of DNA helper strands (H1)/HRP functionalized AuNPs-SiO2@Fe3O4 nanospheres would combine with the hybridization-free TDNs due to the hybridization of H1 and TDNs. The more AFB1 existed in the solution, the more H1/HRP-AuNPs-SiO(2)pFe(3)O(4) could be combined onto the 3DOM MoS2-AuNPs surface. The current response coming from HRP-catalyzed reduction of H2O2 using thionine (Thi) as electrochemical probe was proportional with the AFB1 concentration. Upon optimal conditions, the aptasensor showed specificity for AFB1, achieving a good linear range of 0.1 fg/mL-0.1 mu g/mL and the detection limit of 0.01 fg/mL. Furthermore, the developed aptasensor was also applied for detecting AFB1 content in rice and wheat powder samples, obtaining good results in conformity with those achieved from the high-performance liquid chromatography tandem mass spectrometry (HPLC-MS) method.