Germ Cell Intercellular Bridges
Germ Cell Intercellular Bridges
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DOI：
10.1101/cshperspect.a005850
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发表时间：
2011-08-01
影响因子：
7.2
通讯作者：
Matzuk, Martin M.
中科院分区：
文献类型：
--
作者：
Greenbaum, Michael P.;Iwamori, Tokuko;Matzuk, Martin M.
稳定的细胞间桥是100多年前观察到的多细胞动物配子发生的保守特征，但其功能尚不清楚。通过对果蝇胞质分裂的研究，已经确定了这种结构所必需的许多成分;然而，哺乳动物的细胞间桥具有与昆虫不同的特性。哺乳动物生殖细胞胞间桥由一般胞质分裂组分和包括TEX 14在内的额外生殖细胞特异性因子组成。TEX 14是一种失活的激酶，对维持两性配子中稳定的细胞间桥至关重要，但其缺失特异性地损害雄性减数分裂。TEX 14通过竞争必需组分CEP 55，阻断其在非内皮细胞中与阿利克斯和TSG 101的相互作用，从而阻止凋亡的末端步骤。此外，TEX 14相互作用蛋白RBM 44，其定位在稳定的细胞间桥仅限于粗线期和次级精母细胞，可能参与过程，如RNA运输，但是不必要的细胞间桥的稳定性的维护。
Stable intercellular bridges are a conserved feature of gametogenesis in multicellular animals observed more than 100 years ago, but their function was unknown. Many of the components necessary for this structure have been identified through the study of cytokinesis in Drosophila; however, mammalian intercellular bridges have distinct properties from those of insects. Mammalian germ cell intercellular bridges are composed of general cytokinesis components with additional germ cell-specific factors including TEX14. TEX14 is an inactive kinase essential for the maintenance of stable intercellular bridges in gametes of both sexes but whose loss specifically impairs male meiosis. TEX14 acts to impede the terminal steps of abscission by competing for essential component CEP55, blocking its interaction in nongerm cells with ALIX and TSG101. Additionally, TEX14-interacting protein RBM44, whose localization in stabile intercellular bridges is limited to pachytene and secondary spermatocytes, may participate in processes such as RNA transport but is nonessential to the maintenance of intercellular bridge stability.