A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice.
A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice.
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DOI：
10.1038/ncomms15261
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发表时间：
2017-05-16
影响因子：
16.6
通讯作者：
Shah RN
中科院分区：
文献类型：
--
作者：
Laronda MM;Rutz AL;Xiao S;Whelan KA;Duncan FE;Roth EW;Woodruff TK;Shah RN
新兴的增材制造技术可以研究孔隙几何形状对细胞行为和功能的影响。在这里，我们 3D 打印微孔水凝胶支架，以测试通过操纵打印层之间的前进角度来实现变化的孔隙几何形状如何影响卵巢卵泡的存活。 30° 和 60° 支架提供多侧围绕毛囊的角，而 90° 支架具有开放孔隙，限制了毛囊与支架的相互作用。随着支架相互作用量的增加，卵泡的扩散受到限制并且存活率增加。当植入经过手术绝育的小鼠体内时，卵泡播种支架变得高度血管化，卵巢功能完全恢复。此外，幼崽通过自然交配出生，并通过母亲哺乳而茁壮成长。这些发现提出了一种通过 3D 打印设计的体内功能性卵巢植入物，并表明支架孔隙结构是功能性组织工程增材制造支架设计中的关键变量。临床需要开发支持卵巢移植的生物工程系统。在这里，作者使用不同孔隙结构的 3D 打印支架生成生物假体卵巢，以支持绝育小鼠的卵泡存活和卵巢功能。
Emerging additive manufacturing techniques enable investigation of the effects of pore geometry on cell behavior and function. Here, we 3D print microporous hydrogel scaffolds to test how varying pore geometry, accomplished by manipulating the advancing angle between printed layers, affects the survival of ovarian follicles. 30° and 60° scaffolds provide corners that surround follicles on multiple sides while 90° scaffolds have an open porosity that limits follicle–scaffold interaction. As the amount of scaffold interaction increases, follicle spreading is limited and survival increases. Follicle-seeded scaffolds become highly vascularized and ovarian function is fully restored when implanted in surgically sterilized mice. Moreover, pups are born through natural mating and thrive through maternal lactation. These findings present an in vivo functional ovarian implant designed with 3D printing, and indicate that scaffold pore architecture is a critical variable in additively manufactured scaffold design for functional tissue engineering. There is a clinical need to develop a bioengineering system to support ovary transplantation. Here, the authors generate a bioprosthetic ovary using 3D printed scaffolds of varying pore architectures to support follicle survival and ovarian function in sterilized mice.