cDNA sequence of a new chicken embryonic rho-globin.
cDNA sequence of a new chicken embryonic rho-globin.
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新鸡胚红珠蛋白的 cDNA 序列。
DOI：
10.1073/pnas.78.8.4782
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发表时间：
1981
影响因子：
11.1
通讯作者：
Ingram,VM
中科院分区：
文献类型：
--
作者：
Roninson,IB;Ingram,VM
为了使用特定的DNA探针来研究发育调控基因表达，我们通过使用poly(dG)和poly(dC)接头将cDNA.mRNA杂合体插入质粒pBR322的Pst I位点，制备了与鸡胚胎珠蛋白相对应的cDNA克隆。一个克隆的插入片段的核苷酸序列代表了胚胎β样珠蛋白cDNA的近全长拷贝，已被确定。除了靠近羧基末端的四个氨基酸残基之外，由该插入片段编码的珠蛋白的氨基酸序列与胚胎rho-珠蛋白的序列相同。胚胎和成年鸡 β-珠蛋白 mRNA 序列的比较表明胚胎基因 3' 非翻译区存在大量缺失。
In order to use specific DNA probes for the study of developmentally regulated gene expression, we have prepared cDNA clones corresponding to chicken embryonic globins by inserting cDNA.mRNA hybrids into the Pst I site of the plasmid pBR322 by using poly(dG) and poly(dC) linkers. The nucleotide sequence of the insert of one clone, representing a nearly full-length copy of an embryonic beta-like globin cDNA, has been determined. The amino acid sequence of the globin encoded by this insert is identical to the sequence of embryonic rho-globin, except for four amino acid residues near the carboxy terminus. Comparison of mRNA sequences of the embryonic and adult chicken beta-globins indicates the presence of extensive deletions in the 3' untranslated region of the embryonic gene.