Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens
Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens
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DOI：
10.1038/s41551-017-0084
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发表时间：
2017-07-01
影响因子：
28.1
通讯作者：
Liu, Jonathan T. C.
中科院分区：
文献类型：
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作者：
Glaser, Adam K.;Reder, Nicholas P.;Liu, Jonathan T. C.
对于美国每年接受新癌症诊断的170万患者来说，治疗决定在很大程度上是基于组织病理学标本检查。不幸的是，基于玻片的显微病理学的黄金标准受到观察者之间高度的变异性和有限的预后价值的影响，这是由于采样的限制，以及无法在自然的3D环境中可视化组织结构和分子靶点。在这里，我们展示了一种针对临床标本的非破坏性无载玻片病理而优化的开顶式光片显微镜，使完整组织能够在大的2D和3D视野内以高分辨率快速成像，并具有与传统病理学相同的细节水平。我们展示了这项技术在各种应用中的实用性：广域表面显微镜对外科标本(具有类似200微米表面不规则性)进行分类，术中快速评估肿瘤边缘表面(12.5 S厘米(-2))，以及光学清除的芯针活检的体积评估(直径1毫米，长度2厘米)。光片显微镜对于人体标本的快速表面显微镜和深度体积显微镜都是一种通用的工具。
For the 1.7 million patients per year in the US who receive a new cancer diagnosis, treatment decisions are largely based on histopathological specimen examinations. Unfortunately, the gold standard of slide-based microscopic pathology suffers from high inter-observer variability and limited prognostic value due to sampling limitations and the inability to visualize tissue structures and molecular targets in their native 3D context. Here, we show that an open-top light-sheet microscope optimized for non-destructive slide-free pathology of clinical specimens enables the rapid imaging of intact tissues at high resolution over large 2D and 3D fields of view, with the same level of detail as traditional pathology. We demonstrate the utility of this technology for various applications: wide-area surface microscopy to triage surgical specimens (with similar to 200 mu m surface irregularities), rapid intraoperative assessment of tumour-margin surfaces (12.5 s cm(-2)), and volumetric assessment of optically cleared core-needle biopsies (1 mm in diameter, 2 cm in length). Light-sheet microscopy can be a versatile tool for both rapid surface microscopy and deep volumetric microscopy of human specimens.