MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88
MMP2 and MMP9 are Associated with Apical Periodontitis Progression and Might be Modulated by TLR2 and MyD88
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DOI：
10.1590/0103-6440201801731
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发表时间：
2018-02-01
影响因子：
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通讯作者：
Silva, Raquel Assed Bezerra
中科院分区：
文献类型：
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作者：
Barreiros, Driely;Nelson Filho, Paulo;Silva, Raquel Assed Bezerra
本研究的目的是比较TLR2(TLR2 KO)和MyD88(MyD88 KO)基因敲除小鼠和野生型(WT)小鼠在根尖周炎(AP)进展过程中MMP2和MMP9的表达。TLR2 KO(n=18)、MyD88 KO(n=18)和WT小鼠(n=18)下颌第一磨牙均可诱发AP。7、21和42天后，对动物实施安乐死，解剖颌骨并进行组织技术处理。随后的切片进行免疫组织化学染色，检测MMP2和MMP9的表达。免疫组织化学半定量分析采用卡方检验(α=0.05)。在AP进展的最初阶段，MMP9在TLR2 KO和MyD88 KO小鼠中的表达增加。在AP进展的最后阶段，TLR2 KO小鼠MMP2表达减少，MMP9表达增加。MMP2和MMP9的产生受TLR2和MyD88的调控。
The aim of this study was to evaluate the expression of MMP2 and MMP9 during apical periodontitis (AP) progression in TLR2 (TLR2 KO) and in MyD88 (MyD88 KO) knockout mice compared to wild type (WT) mice. AP was induced in mandibular first molars of TLR2 KO (n= 18), MyD88 KO (n= 18), and WT mice (n= 18). After 7, 21, and 42 days, the animals were euthanized and the jaws were dissected and subjected to histotechnical processing. Subsequent sections were stained by immunohistochemistry and evaluated for detection of MMP2 and MMP9. Statistical analysis of the semi-quantitative analysis of immunohistochemistry was performed using chi-square test (alpha = 0.05). In the initial periods of AP progression, an increased expression of MMP9 in the TLR2 KO and MyD88 KO mice was observed. In the final periods of AP progression, a reduction of MMP2 expression and an increase of MMP9 expression in the TLR2 KO mice were observed. MMP2 and MMP9 production was modulated for TLR2 and MyD88 during apical periodontitis progression.