Absorption and excretion of conjugated flavonols, including quercetin-4′-O-β-glucoside and isorhamnetin-4′-O-β-glucoside by human volunteers after the consumption of onions
Absorption and excretion of conjugated flavonols, including quercetin-4′-O-β-glucoside and isorhamnetin-4′-O-β-glucoside by human volunteers after the consumption of onions
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DOI：
10.1080/10715769800300291
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发表时间：
1998-01-01
影响因子：
3.3
通讯作者：
Crozier, A
中科院分区：
文献类型：
--
作者：
Aziz, AA;Edwards, CA;Crozier, A
黄酮醇是普遍存在于植物和植物产品中的多酚。黄酮醇，特别是槲皮素，是体外有效的抗氧化剂，其摄入量与冠心病的发病率呈负相关。本研究的目的是调查血浆中的积累和尿液中的黄酮醇糖苷摄入轻度油炸洋葱后排泄。五名健康志愿者遵循低类黄酮饮食3天。在第4天，禁食过夜后，给予受试者300 g轻度油炸的黄色洋葱，其含有槲皮素和异鼠李素的缀合物，包括槲皮素-3，4 '-二O-β-葡糖苷、异鼠李素-4'-O-β-葡糖苷和槲皮素-4 '-O-β-葡糖苷。在补充后0分钟、0.5、1.0、1.5、2、3、4、5和24小时进行血液收集。此外，受试者在补充洋葱后24小时收集所有尿液。异鼠李素-4 '-O-β-葡萄糖苷和槲皮素-4'-O-β-葡萄糖苷在血浆中蓄积，最大水平（定义为摄入比例）分别为10.7 +/- 2.6%和0.13 +/- 0.03%。摄入洋葱后，槲皮素-4 '-葡萄糖苷的血浆峰浓度时间为1.3 +/- 0.2 h，而异鼠李素-4'-葡萄糖苷的血浆峰浓度值为1.8 +/- 0.7 h。异鼠李素-4 '-O-β-葡萄糖苷和槲皮素-4'-O-β-葡萄糖苷的尿液排泄（占摄入量的比例）分别为17.4 +/- 8.3%和0.2 +/- 0.1%。异鼠李素-4 '-葡萄糖苷的积累和排泄比例远高于槲皮素-4'-葡萄糖苷的可能原因进行了讨论。它的结论是，黄酮醇被吸收到血液中的葡萄糖苷和轻微的结构差异显着影响的积累水平和共轭物被排泄的程度。
Flavonols are polyphenols found ubiquitously in plants and plant-products. Flavonols, particularly quercetin, are potent antioxidants in vitro and their intake has been associated inversely with the incidence of coronary heart disease. The aim of this study was to investigate the accumulation in plasma and excretion in urine of flavonol glucosides following ingestion of lightly fried onions. Five healthy volunteers followed a low-flavonoid diet for 3 days. On day 4, after an overnight fast, subjects were given 300 g of lightly fried yellow onions which contain conjugates of quercetin and isorhamnetin, including quercetin-3,4'-diO-beta-glucoside, isorhamnetin-4'-O-beta-glucoside and quercetin-4'-O-beta-glucoside. Blood collection was carried out at 0 min, 0.5, 1.0, 1.5, 2, 3, 4, 5 and 24 h after the supplement. In addition, subjects collected all their urine for 24 h following the onion supplement. Isorhamnetin-4'-O-beta-glucoside and quercetin-4'-O-beta-glucoside accumulated in plasma with maximum levels, defined as proportion of intake, of 10.7 +/- 2.6% and 0.13 +/- 0.03% respectively. The time of the quercetin-4'-glucoside peak plasma concentration was 1.3 +/- 0.2 h after the ingestion of onions while a value of 1.8 +/- 0.7 h was obtained for isorhamnetin-4'-glucoside. Excretion in urine, as a proportion of intake, was 17.4 +/- 8.3% for isorhamnetin-4'-O-beta-glucoside and 0.2 +/- 0.1% for quercetin-4'-O-beta-glucoside. Possible reasons for the accumulation and excretion of isorhamnetin-4'-glucoside in proportionally much higher amounts than quercetin-4'-glucoside are discussed. It is concluded that flavonols are absorbed into the bloodstream as glucosides and minor structural differences affect markedly both the level of accumulation and the extent to which the conjugates are excreted.