Effects of acute γ-irradiation on community structure of the aquatic microbial microcosm
Effects of acute γ-irradiation on community structure of the aquatic microbial microcosm
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DOI：
10.1016/j.jenvrad.2010.06.007
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发表时间：
2010-11-01
影响因子：
2.3
通讯作者：
Inamori, Yuhei
中科院分区：
文献类型：
--
作者：
Fuma, Shoichi;Ishii, Nobuyoshi;Inamori, Yuhei
为了阐明电离辐射对水生微生物群落的间接影响，在由绿色（Chlorella sp.和Scenedesmus sp.）和蓝绿单歧藻（Tolypothrix sp.）作为生产者：纤毛虫原生动物（Cyclidium glaucoma）、轮虫（Lecane sp.和Philodina sp.）和一种寡毛纲动物（Aeolosoma hemprichi）作为消费者;以及四种以上的细菌作为分解者。在辐照后160天内观察到组成生物体的种群变化。利用165 rDNA的变性梯度凝胶电泳（DGGE）分析了该菌的群落结构。原理响应曲线分析表明，作为一个整体的微观世界的人口没有显着影响，在100戈伊，而他们受到不利影响，在500-5000戈伊剂量依赖性的方式。然而，对每个种群的一些影响，包括DGGE检测到的每个细菌种群，并不依赖于辐射剂量，在辐照微观世界中的一些种群大于对照。这些意想不到的结果被认为是通过种间相互作用的间接影响，并提出了可能的机制，起源于在微观世界中共存的其他生物体的人口变化。例如，对消费者和分解者的一些间接影响可能来自每个营养级内的种间竞争。生产者和消费者之间的猎物-捕食者关系也可能对生产者造成一些间接影响。(C)2010爱思唯尔有限公司保留所有权利。
To characterise indirect effects of ionising radiation on aquatic microbial communities, effects of acute gamma-irradiation were investigated in a microcosm consisting of populations of green algae (Chlorella sp. and Scenedesmus sp.) and a blue-green alga (Tolypothrix sp.) as producer: a ciliate protozoan (Cyclidium glaucoma), rotifers (Lecane sp. and Philodina sp.) and an oligochaete (Aeolosoma hemprichi) as consumer; and more than four species of bacteria as decomposers. Population changes in the constituent organisms were observed over 160 days after irradiation. Prokaryotic community structure was also examined by denaturing gradient gel electrophoresis (DGGE) of 165 rDNA. Principle response curve analysis revealed that the populations of the microcosm as a whole were not significantly affected at 100 Gy while they were adversely affected at 500-5000 Gy in a dose-dependent manner. However, some effects on each population, including each bacterial population detected by DGGE, did not depend on radiation doses, and some populations in the irradiated microcosm were larger than those of the control. These unexpected results are regarded as indirect effects through interspecies interactions, and possible mechanisms are proposed originating from population changes in other organisms co-existing in the microcosm. For example, some indirect effects on consumers and decomposers likely arose from interspecies competition within each trophic level. It is also likely that prey predator relationships between producers and consumers caused some indirect effects on producers. (C) 2010 Elsevier Ltd. All rights reserved.