Comparative Proteomics Indicates That Biosynthesis of Pectic Precursors Is Important for Cotton Fiber and Arabidopsis Root Hair Elongation
Comparative Proteomics Indicates That Biosynthesis of Pectic Precursors Is Important for Cotton Fiber and Arabidopsis Root Hair Elongation
复制标题
DOI：
10.1074/mcp.m110.000349
复制
发表时间：
2010-09-01
影响因子：
7
通讯作者：
Zhu, Yu-Xian
中科院分区：
文献类型：
--
作者：
Pang, Chao-You;Wang, Hui;Zhu, Yu-Xian
棉纤维的质量取决于其最终长度和强度，这是初生和次生细胞壁沉积的函数。使用比较蛋白质组学方法，我们确定了104个蛋白质从棉花胚珠开花后10天，93个优先积累在野生型和11个积累在无绒毛无绒突变体。生物信息学分析表明，核苷酸糖代谢是纤维伸长过程中最显著上调的生化过程。七个蛋白质点可能参与果胶细胞壁多糖生物合成的野生型样品中的蛋白质和转录水平的具体积累。当向培养基中添加乙烯或木蜡酸（C24：0）时，这些基因的蛋白质和mRNA表达增加，表明这些化合物可能通过调节细胞壁聚合物的产生来促进纤维伸长。定量分析表明，纤维初生细胞壁含有显着较高的果胶量，而更多的半纤维素被发现在胚珠样品。显著的纤维生长时观察到UDP-L-鼠李糖，UDP-D-半乳糖醛酸，或UDP-D-葡萄糖醛酸，所有这些都很容易纳入果胶部分的细胞壁制剂，被添加到胚珠培养基。拟南芥uer 1 -1和gae 6 -1突变体的短根毛的补充，无论是通过遗传转化各自的棉花cDNA或通过添加特定的果胶前体的生长培养基。当两种果胶前体，无论是UDP-4-酮-6-脱氧-D-葡萄糖3，5-差向异构酶4-还原酶或UDP-D-葡萄糖脱氢酶和UDP-D-葡萄糖醛酸4-差向异构酶先后产生的，在化学互补试验中，观察到野生型根毛长度在两个cut 1和ein 2 -5拟南芥幼苗，这表明C24：0生物合成或乙烯信号的缺陷，分别。乙烯和C24：0可能通过激活果胶合成网络，尤其是UDP-L-鼠李糖和UDP-D-半乳糖醛酸的合成，促进棉花纤维和拟南芥根毛的生长。Molecular & Cellular Proteomics 9：2019-2033，2010.
The quality of cotton fiber is determined by its final length and strength, which is a function of primary and secondary cell wall deposition. Using a comparative proteomics approach, we identified 104 proteins from cotton ovules 10 days postanthesis with 93 preferentially accumulated in the wild type and 11 accumulated in the fuzzless-lintless mutant. Bioinformatics analysis indicated that nucleotide sugar metabolism was the most significantly upregulated biochemical process during fiber elongation. Seven protein spots potentially involved in pectic cell wall polysaccharide biosynthesis were specifically accumulated in wild-type samples at both the protein and transcript levels. Protein and mRNA expression of these genes increased when either ethylene or lignoceric acid (C24:0) was added to the culture medium, suggesting that these compounds may promote fiber elongation by modulating the production of cell wall polymers. Quantitative analysis revealed that fiber primary cell walls contained significantly higher amounts of pectin, whereas more hemicellulose was found in ovule samples. Significant fiber growth was observed when UDP-L-rhamnose, UDP-D-galacturonic acid, or UDP-D-glucuronic acid, all of which were readily incorporated into the pectin fraction of cell wall preparations, was added to the ovule culture medium. The short root hairs of Arabidopsis uer1-1 and gae6-1 mutants were complemented either by genetic transformation of the respective cotton cDNA or by adding a specific pectin precursor to the growth medium. When two pectin precursors, produced by either UDP-4-keto-6-deoxy-D-glucose 3,5-epimerase 4-reductase or by UDP-D-glucose dehydrogenase and UDP-D-glucuronic acid 4-epimerase successively, were used in the chemical complementation assay, wild-type root hair lengths were observed in both cut1 and ein2-5 Arabidopsis seedlings, which showed defects in C24:0 biosynthesis or ethylene signaling, respectively. Our results suggest that ethylene and C24:0 may promote cotton fiber and Arabidopsis root hair growth by activating the pectin biosynthesis network, especially UDP-L-rhamnose and UDP-D-galacturonic acid synthesis. Molecular & Cellular Proteomics 9:2019-2033, 2010.