CORTISOL INHIBITION OF DEVELOPMENT OF VARIOUS LYSOSOMAL-ENZYMES IN CULTURED PALATAL SHELVES FROM MOUSE EMBRYOS
CORTISOL INHIBITION OF DEVELOPMENT OF VARIOUS LYSOSOMAL-ENZYMES IN CULTURED PALATAL SHELVES FROM MOUSE EMBRYOS
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DOI：
10.1016/0003-9969(83)90167-x
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发表时间：
1983-01-01
影响因子：
3
通讯作者：
HEROLD, R
中科院分区：
文献类型：
--
作者：
ADS, AH;PIDDINGTON, R;HEROLD, R
[糖皮质激素在实验中与类固醇敏感小鼠品系胎仔中继发腭裂的高发生率有关。]溶酶体酶、非特异性酯酶、β-葡糖醛酸酶，N-乙酰基-β-用组织化学方法检测了体外培养的单个腭突内侧缘上皮细胞（medial-edge epithelium，EEP）中氨基葡萄糖苷酶（glucosaminidase）和芳基硫酸酯酶（arylsulfatases）A和B。大多数酶活性在培养过程中逐渐升高，在培养24-26 h达到高峰。在整个培养过程中，腭突细胞中芳基硫酸酯酶B活性较低，芳基硫酸酯酶A活性未检测到。48 h时，细胞变性消失。皮质醇抑制这些水解酶的活性增加，并防止程序性腭上皮破裂。
[Glucocorticoids have been implicated experimentally in the production of high incidences of clefts of the secondary palate in fetuses from steroid-sensitive mouse strains.] The lysosomal enzymes, nonspecific esterases, .beta.-glucuronidase, N-acetyl-.beta.-glucosaminidase and arylsulfatases A and B, were examined histochemically in medial-edge epithelia (MEE) of single palatal shelves in vitro. Activities of most enzymes increased gradually in MEE with a peak at 24-26 h of culture. Arylsulfatase B activities were lower than the others and arylsulfatase A activities could not be detected in the palatal cells during the entire culture period. By 48 h, MEE cells degenerated and were lost. Cortisol suppressed increased activities of these hydrolytic enzymes and prevented programmed breakdown of the palatal epithelium.