MultiBac: from protein complex structures to synthetic viral nanosystems.
MultiBac: from protein complex structures to synthetic viral nanosystems.
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DOI：
10.1186/s12915-017-0447-6
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发表时间：
2017-10-30
期刊：
影响因子：
5.4
通讯作者：
Berger I
中科院分区：
文献类型：
--
作者：
Pelosse M;Crocker H;Gorda B;Lemaire P;Rauch J;Berger I
MultiBac 杆状病毒/昆虫细胞表达载体系统被认为是一种用户友好的模块化工具包，用于生产用于结构生物学应用的多蛋白复合物。 MultiBac 使许多分子机器的结构和功能得以阐明，包括以前无法接近的高价值药物靶点。最近，MultiBac 的开发已转向定制杆状病毒基因组，这些基因组是为一系列应用量身定制的，包括通过遗传密码扩展合成人工蛋白质。我们回顾了其中的一些进展，包括正在进行的用于哺乳动物应用的 MultiBac 系统的重新布线，特别是 CRISPR/Cas9 介导的基因编辑。
The MultiBac baculovirus/insect cell expression vector system was conceived as a user-friendly, modular tool-kit for producing multiprotein complexes for structural biology applications. MultiBac has allowed the structure and function of many molecular machines to be elucidated, including previously inaccessible high-value drug targets. More recently, MultiBac developments have shifted to customized baculoviral genomes that are tailored for a range of applications, including synthesizing artificial proteins by genetic code expansion. We review some of these developments, including the ongoing rewiring of the MultiBac system for mammalian applications, notably CRISPR/Cas9-mediated gene editing.