Isolation of deoxyribonucleic acid (DNA) from saliva and forensic science samples containing saliva.
Isolation of deoxyribonucleic acid (DNA) from saliva and forensic science samples containing saliva.
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从唾液和含有唾液的法医学样本中分离脱氧核糖核酸 (DNA)。
DOI：
10.1520/jfs13248j
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发表时间：
1992
影响因子：
1.6
通讯作者：
Daniel D. Garner
中科院分区：
文献类型：
--
作者：
D. J. Walsh;Amy C. Corey;Robin W. Cotton;Lisa Forman;G. Herrin;Charlotte J. Word;Daniel D. Garner
将唾液和唾液染色材料作为脱氧核糖核酸（DNA）的潜在来源进行检查，以进行DNA分析和鉴别检测。在本文中，作者证明，DNA的分离和DNA带型适用于DNA分型，从新鲜的唾液和各种唾液染色的材料，如信封，口腔拭子，插科打诨，香烟。此外，从含有混合唾液/精液污渍的实际法医证据样本中获得DNA和DNA带型。从唾液或唾液染色材料中获得的DNA带型与从同一个体的血液或毛发中获得的带型无法区分。完整的DNA很容易分离，并从储存在-20 ℃的唾液和储存在不同条件下的干燥唾液污渍中获得DNA带型。我们的结论是，唾液和唾液染色的材料可以是很好的DNA分析和DNA分型在某些法医设置的来源。
Saliva and saliva-stained materials were examined as potential sources of deoxyribonucleic acid (DNA) for DNA analysis and identity testing. In this paper, the authors demonstrate that DNA was isolated and DNA banding patterns suitable for DNA typing were obtained from fresh saliva and various saliva-stained materials, such as envelopes, buccal swabs, gags, and cigarettes. Furthermore, DNA and DNA banding patterns were obtained from actual forensic evidentiary samples containing mixed saliva/semen stains. The DNA banding patterns obtained from saliva or saliva-stained material were indistinguishable from the patterns obtained from blood or hair from the same individual. Intact DNA was readily isolated and DNA banding patterns were obtained from saliva stored at -20 degrees C and dried saliva stains stored under varying conditions. We conclude that saliva and saliva-stained material can be good sources of DNA for analysis and for DNA typing in certain forensic settings.