Identity of a Chi site of Escherichia coli and Chi recombinational hotspots of bacteriophage lambda.
Identity of a Chi site of Escherichia coli and Chi recombinational hotspots of bacteriophage lambda.
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大肠杆菌的 Chi 位点和 lambda 噬菌体的 Chi 重组热点的身份。
DOI：
10.1016/0022-2836(82)90072-9
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发表时间：
1982
影响因子：
5.6
通讯作者：
Smith,GR
中科院分区：
文献类型：
--
作者：
Triman,KL;Chattoraj,DK;Smith,GR
λ噬菌体中的Chi位点刺激大肠杆菌RecBC途径促进的重组。我们在E. coli lacZ基因的缺失定位，并分离到一个使该基因失活的突变。序列分析表明，该突变是由一个8个碱基对的序列（5′ GCTGGTGG 3′）中的一个碱基对G C今→ A T今的转换引起的，与λ和质粒pBR 322中的Chi位点完全相同。
Chi sites in bacteriophage λ stimulate recombination promoted by the RecBC pathway of Escherichia coli. We have located a Chi site within the E. coli lacZ gene by deletion mapping and have isolated a mutation inactivating this Chi. Sequence analysis showed that the mutation arose by a single base-pair transition G C ̇→ A T ̇ within an eight base-pair sequence (5′ GCTGGTGG 3′) identical to that found at Chi sites in λ and in plasmid pBR322.