Human telomerase reverse transcriptase expression in colorectal tumors: correlations with immunohistochemical expression and clinicopathologic features
Human telomerase reverse transcriptase expression in colorectal tumors: correlations with immunohistochemical expression and clinicopathologic features
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DOI：
10.1016/j.anndiagpath.2010.06.007
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发表时间：
2010-12-01
影响因子：
2
通讯作者：
Karaoglu, Aziz
中科院分区：
文献类型：
--
作者：
Simsek, Bengu Cobanoglu;Pehlivan, Sultan;Karaoglu, Aziz
人端粒酶逆转录酶（hTERT）蛋白在结直肠癌中的研究在几项研究中，但据我们所知，hTERT的表达还没有在所有的结直肠肿瘤，包括增生性息肉（HP），腺瘤，癌，石蜡包埋的组织切片进行评估。本研究的目的是探讨免疫组化hTERT表达及其与临床病理特征的关系，在一系列的结直肠肿瘤。本研究采用免疫组化方法检测了20例HP、20例腺瘤性息肉（AP）、20例大肠腺癌和20例正常大肠粘膜中hTERT的表达。结果与临床病理特征相关。hTERT在腺瘤和癌组织中的表达水平明显高于正常组织（P <0.05）。HP与AP之间也有显著性差异（P <0.05）。癌组织中hTERT表达高于腺瘤组织，但差异无统计学意义（P > 0.05）。hTERT在癌、癌前病变或正常粘膜中的表达与年龄、性别和病变大小等临床病理参数无显著相关性（P > 0.05），但仅与癌的组织学分级相关（P <0.05）。免疫组化结果显示hTERT在正常胃粘膜和HP中表达极少，在AP中表达中等，在癌中表达最高。因此，hTERT的表达可以作为大肠肿瘤侵袭性的标志物，但它与临床病理数据无关。(C)2010年爱思唯尔公司All rights reserved.
Human telomerase reverse transcriptase (hTERT) proteins in colorectal cancer investigated in several studies, but to our knowledge, hTERT expression has not been evaluated in all of colorectal tumors, including hyperplastic polyps (HPs), adenomas, and carcinomas, on paraffin-embedded tissue sections. The aim of the present study is to investigate immunohistochemical hTERT expression and its relationship with the clinicopathologic features in a spectrum of colorectal tumors. In this study, hTERT expression was determined in HP (n = 20), adenomatous polyp (AP) (n = 20), colorectal adenocarcinomas (n = 20), and normal mucosa (n = 20) by immunohistochemical method. The findings were correlated with the clinicopathologic features. The staining level of hTERT in adenomas and carcinomas was significantly higher than in normal tissues (P < .05). There was also significant difference between HP and AP (P < .05). Level of hTERT in carcinomas was higher than in adenomas, but the difference was of no statistical significance (P > .05). There was no significant association of hTERT expression in cancerous, precancerous, or normal mucosa related to clinicopathologic parameters including age, sex, and size of lesion, (P > .05), but only association with histologic grade for carcinoma was found (P < .05). Levels of hTERT by immunohistochemistry demonstrated that the expression of hTERT was very little in normal mucosa and HP, moderate in AP, and highest in carcinoma. Thereby, hTERT expression may use the aggressiveness of the colorectal tumors as a marker, but it is not related to clinicopathologic data. (C) 2010 Elsevier Inc. All rights reserved.