Identification of four caspase genes from Spodoptera exigua (Lepidoptera: Noctuidae) and their regulations toward different apoptotic stimulations
Identification of four caspase genes from Spodoptera exigua (Lepidoptera: Noctuidae) and their regulations toward different apoptotic stimulations
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甜菜夜蛾（鳞翅目：夜蛾科）四种 caspase 基因的鉴定及其对不同细胞凋亡刺激的调控
DOI：
10.1111/1744-7917.12741
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发表时间：
2020
期刊：
影响因子：
4
通讯作者：
Guo-Hua Huang
中科院分区：
文献类型：
--
作者：
Huan Yu;Zi-Qi Li;Yi-Yi Ou-Yang;Guo-Hua Huang
细胞凋亡在多细胞生物的多种生物学过程中起着重要作用。Caspase是细胞凋亡的重要参与者和调节者。本研究克隆了甜菜夜蛾（Spodoptera exigua）4个新的caspase基因，分别命名为SeCasp-1、SeCasp-6、SeCasp-7和SeCasp-8。对这些SeCasp的推定编码蛋白质序列的分析表明，SeCasp-1和SeCasp-7可能是执行caspase的同源物; SeCasp-8可能是起始caspase的同源物; SeCasp-6是S的独特caspase。与所有鉴定的昆虫半胱天冬酶具有低相似性。基于杆状病毒表达系统分析，SeCasp-1表现出与人半胱天冬酶-1、-3、-4、-6、-8和-9相似的半胱天冬酶活性; SeCasp-6表现出与人半胱天冬酶-2、-3、-4、-6、-8和-9相似的半胱天冬酶活性; SeCasp-7表现出与人半胱天冬酶-2、-3和-6相似的半胱天冬酶活性; SeCasp-8仅表现出与人半胱天冬酶-8相似的半胱天冬酶活性。用不同的化学物质诱导显示，SeCasp-1在处理的S. exigua.发育表达分析显示，SeCasp-1在幼虫期高度转录，而SeCasp-6，SeCasp-7，SeCasp-8则下调。体内检测S.不同病原菌对棉铃虫幼虫的感染试验表明，SeCasp-1对苏云金芽孢杆菌敏感，SeCasp-6对杆状病毒敏感。SeCasp-7和SeCasp-8在体外化学凋亡诱导或体内病原体感染下均显示轻微变化。
Apoptosis plays critical roles in multiple biological processes in multicellular organisms. Caspases are known as important participators and regulators of apoptosis. Here, four novel caspase genes of Spodoptera exigua were cloned and characterized, which were designated as SeCasp‐1, SeCasp‐6, SeCasp‐7 and SeCasp‐8. Analysis of the putative encoded protein sequences of these SeCasps indicated that SeCasp‐1 and SeCasp‐7 were possible homologs of executor caspases; SeCasp‐8 was a possible homolog of initiator caspases; and SeCasp‐6 was a unique caspase of S. exigua that shares low similarity with all the identified insect caspases. Based on baculovirus expression system analyses, SeCasp‐1 exhibited similar caspase activity to human caspase‐1, ‐3, ‐4, ‐6, ‐8 and ‐9; SeCasp‐6 presented similar caspase activity to human caspase‐2, ‐3, ‐4, ‐6, ‐8 and ‐9; SeCasp‐7 exhibited similar caspase activity to human caspase‐2, ‐3 and ‐6; and SeCasp‐8 presented similar caspase activity only to human caspase‐8. Induction with different chemicals revealed that SeCasp‐1 showed extreme upregulation after 24 h in the treated fat body cell line (IOZCAS‐Spex‐II) of S. exigua. Developmental expression analysis revealed that SeCasp‐1 was highly transcribed in the larval stages, while SeCasp‐6, SeCasp‐7, SeCasp‐8 were down‐regulated. The in vivo detection of the relative expression levels of SeCasps in S. eixgua larvae inoculated with different pathogens suggested that SeCasp‐1 was sensitive to Bacillus thuringiensis infection and that SeCasp‐6 was sensitive to baculovirus infection. SeCasp‐7 and SeCasp‐8 showed slight changes under either in vitro chemical apoptosis induction or in vivo pathogen infection.