Comparative molecular mapping in Ceratotropis species using an interspecific cross between azuki bean (Vigna angularis) and rice bean (V-umbellata)
Comparative molecular mapping in Ceratotropis species using an interspecific cross between azuki bean (Vigna angularis) and rice bean (V-umbellata)
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DOI：
10.1007/s001220050028
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发表时间：
2000-01-01
影响因子：
5.4
通讯作者：
Kamijima, O
中科院分区：
文献类型：
--
作者：
Kaga, A;Ishii, T;Kamijima, O
以小豆（Vigna angularis, 2n=2x=22）与稻豆（V. umellata, 2n=2x=22）种间杂交的86株F-2植株为材料，建立了遗传连锁图谱。共构建了14个连锁组，每个连锁组包含4个以上的标记，其中包含1个表型，114个RFLP和74个RAPD标记。图谱总尺寸为1702 cM，标记物之间的平均距离为9.7 cM。显示显著偏离预期比率的位点聚集在几个连锁组中。大多数偏位是由于水稻豆类等位基因的优势。并将该图谱与其他已有的角鼻亚属紫豆属植物图谱进行了比较。基于共同标记的谱系分析，在azuki bean x V. nakashimae种间图谱和绿豆种间图谱中分别发现了7个和16个保守连锁块。虽然目前的图谱还没有完全饱和，但它可以为豇豆育种的基因标记、QTL定位和进一步有用的基因转移提供便利。
A genetic linkage map was developed with 86 F-2 plants derived from an interspecific cross between azuki bean (Vigna angularis, 2n=2x=22) and rice bean (V. umbellata, 2n=2x=22). In total, 14 linkage groups, each containing more than 4 markers, were constructed with one phenotypic, 114 RFLP and 74 RAPD markers. The total map size was 1702 cM, and the average distance between markers was 9.7 cM. The loci showing significant deviation from the expected ratio clustered in several linkage groups. Most of the skewed loci were due to the predominance of rice bean alleles. The azuki-rice bean linkage map was compared with other available maps of Vigna species in subgenus Ceratotropis. Based on the lineage of the common mapped markers, 7 and 16 conserved linkage blocks were found in the interspecific map of azuki bean x V. nakashimae and mungbean map, respectively. Although the present map is not fully saturated, it may facilitate gene tagging, QTL mapping and further useful gene transfer for azuki bean breeding.