Comparison of the two major ARS elements of the ura4 replication origin region with other ARS elements in the fission yeast, Schizosaccharomyces pombe.
Comparison of the two major ARS elements of the ura4 replication origin region with other ARS elements in the fission yeast, Schizosaccharomyces pombe.
复制标题
ura4 复制起点区的两个主要 ARS 元件与裂殖酵母裂殖酵母中其他 ARS 元件的比较。
DOI：
10.1007/bf00362286
复制
发表时间：
1994
期刊：
影响因子：
1.6
通讯作者：
Huberman,JA
中科院分区：
文献类型：
--
作者：
Zhu,J;Carlson,DL;Dubey,DD;Sharma,K;Huberman,JA
We have previously peported that the replication orgin region located near theura4gene on chromosome III of the fission yeast,Schizosaccharomyces pombe, contains three closely spaced origins, each associated with an autonomously replicating sequence (ARS) element. Here we report the nucleotide sequences of two of these ARS elements,ars3002andars3003. The two ARS elements are located on either side of a transcribed 1.5 kb open reading frame. Like 11 otherS. pombeARS elements whose sequences have previously been determined in other laboratories, the 2 new ARS elements are unusually A+T-rich. All 13 ARS elements contain easily unwound stretches of DNA. Each of the ARS elements contains numerous copies, at a higher than expected frequency, of short stretches of A+T-rich DNA in which most of the Ts are on one strand and most of the As are on the complementary strand. We discuss the potential significance for ARS function of these multiple asymmetric A+T-rich sequences.
