Appraising the instantaneous secretory rates of luteinizing hormone and testosterone in response to selective mu opiate receptor blockade in late pubertal boys.
Appraising the instantaneous secretory rates of luteinizing hormone and testosterone in response to selective mu opiate receptor blockade in late pubertal boys.
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评估青春期晚期男孩选择性 mu 阿片受体阻断后黄体生成素和睾酮的瞬时分泌率。
DOI：
10.1002/j.1939-4640.1987.tb03304.x
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发表时间：
1987
影响因子：
--
通讯作者：
Veldhuis,JD
中科院分区：
文献类型：
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作者：
Mauras,N;Rogol,AD;Veldhuis,JD
对4名青春期晚期正常男童(年龄14 8/12~15 1/12岁)用纳曲酮隔日5 0 mg慢性阻断MU阿片受体前后，测定促性腺激素和睾酮释放的脉动特性。使用一种新的离散去卷积算法来估计表观瞬时分泌率，以评估自发分泌事件的性质，包括每20分钟提取一次的血液中免疫活性的黄体生成素和睾酮，持续24小时。该方法的应用表明，阻断Mu阿片受体后，离散型黄体生成素瞬时分泌率增加(P=0.011)。更显著的是，睾酮分泌事件的所有参数对Mu阿片受体阻断都有显著的反应，包括平均估计分泌率(+47%，P=0.02)、睾酮脉冲频率(+%，P<；0.001)和幅度(+20%，P=0.027)。相应地，睾酮分泌间期缩短(-35%，P=0.001)，分泌间期缩短(-19%，P=0.042)，脉搏间期缩短(-35%，P=0.006)。睾酮分泌有明显的昼夜节律，在上午和傍晚达到最大值，下午显著下降，有时降至青春期前水平。睾酮分泌的这种变化与黄体生成素的变化是平行的。纳曲酮作用后，卵泡刺激素平均浓度(+18%)和平均峰值幅度(+15%，P=0.002)均显著升高。这些数据提供了阿片系统与下丘脑GnRH脉冲发生器功能耦合的间接证据。在阿片受体阻断后，黄体生成素和睾酮分泌活性(以瞬时分泌速率衡量)显著增加，这表明在男性青春期后期，内源性阿片系统和下丘脑-脑垂体性腺轴之间存在完整的相互作用。
The pulsatile properties of gonadotropin and testosterone release were examined before and after chronic mu opiate receptor blockade with naltrexone, 50 mg every other day, in four normal boys in late puberty (ages 14 8/12 to 15 1/12 years). The nature of spontaneous secretory events was appraised for immunoactive LH and testosterone in blood withdrawn every 20 minutes for 24 hours, using a novel, discrete deconvolution algorithm to estimate apparent instantaneous secretory rates. The application of this methodology revealed that the frequency of discrete LH instantaneous secretory rates increased after mu opiate receptor blockade (P= 0.011). More strikingly, all parameters of testosterone secretory events responded significantly to mu opiate receptor blockade, including increases in mean estimated secretory rate (+ 47%,P= 0.02), testosterone pulse frequency (+ 64%,P< 0.001) and amplitude (+ 20%,P= 0.027). Correspondingly, decreases in testosterone interpulse secretory intervals (‐ 35%,P= 0.001), secretory pulse duration (‐ 19%,P= 0.042) and interpulse valley duration (‐ 35%,P= 0.006) also were noted. There was a prominent diurnal rhythm in testosterone secretion with maximal values in the morning and late evening, and marked reductions in the afternoon, sometimes to prepubertal levels. This variation in the testosterone secretory profile paralleled that of LH. In response to naltrexone, the FSH concentration series showed a significant increase in the mean FSH concentration (+ 18%)P= 0.003) and mean peak amplitude (+ 15%,P= 0.002). These data provide indirect evidence of functional coupling of the opiate system with the hypothalamic GnRH pulse generator. The marked increase in LH and testosterone secretory activity (as measured by the instantaneous secretory rate) after opiate receptor blockade suggests an integral interaction between the endogenous opiate system and the hypothalamic‐pituitary gonadal axis during the later stages of puberty in males.