Chromosome variability of human multipotent mesenchymal stromal cells
Chromosome variability of human multipotent mesenchymal stromal cells
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DOI：
10.1007/s10517-007-0031-0
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发表时间：
2007-01-01
影响因子：
0.7
通讯作者：
Gol'dshtein, D. V.
中科院分区：
文献类型：
--
作者：
Bochkov, N. P.;Voronina, E. S.;Gol'dshtein, D. V.
We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passage cultures from 2 of 7 individuals. The percent of aberrant cells in cultures from different individuals varied from 1.5 to 5.95 per 100 cells, which attested to karyotype instability. These data substantiate the need for cytogenetic control of cells before their transplantation into donor organism and further investigation of chromosome variability in stem cells.
