Evolution of action potential propagation and repolarization in cultured neonatal rat ventricular myocytes
Evolution of action potential propagation and repolarization in cultured neonatal rat ventricular myocytes
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DOI：
10.1046/j.1540-8167.2001.01269.x
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发表时间：
2001-11-01
影响因子：
2.7
通讯作者：
Binah, O
中科院分区：
文献类型：
--
作者：
Meiry, G;Reisner, Y;Binah, O
培养的新生大鼠心室肌细胞的成熟。简介：培养的新生大鼠心室肌细胞（NRVM）重新建立间隙连接，因为它们形成同步和自发跳动的单层，从而提供了一个有用的模型，用于研究激活和复极。方法和结果：我们使用的多电极阵列数据采集系统与60单极电极，调查培养NRVM的功能组织，通过确定传播和复极模式。根据每个电极的局部激活时间构建激活图。在培养的第3 ~ 8天，QRS振幅和dV/dt（max）随年龄增加而增加。同时，随着培养物的成熟，QT间期（代表动作电位时程）缩短，T波振幅和T波上升和下降肢的斜率进行性增加。传导速度的变化是不同的，比那些电描记特性，略有增加，在第3至5天，并逐渐下降到第8天在culture.Conclusion：建立统一的激活模式自发放电或驱动的单层培养的心肌细胞伴随着组织的激活和复极的演变出现在音乐会与一个成熟的连接蛋白43染色模式。在这项研究中开发的实验技术提供了有用的工具，调查缝隙连接，传导速度和传播模式之间的复杂关系，以及一种手段，以了解在病理生理条件下的缝隙连接重构如何使心肌心律失常。
Maturation of Cultured Neonatal Rat Ventricular Myocytes. Introduction: Cultured neonatal rat ventricular myocytes (NRVM) reestablish gap junctions as they form synchronously and spontaneously beating monolayers, thus providing a useful model for studying activation and repolarization.Methods and Results: We used the multielectrode array data acquisition system with 60 unipolar electrodes to investigate the functional organization of cultured NRVM, by determining propagation and repolarization patterns. Activation maps were constructed from the local activation times at each electrode. During days 3 to 8 in culture, QRS amplitude and dV/dt(max) increased with age. Concomitantly, with the culture maturation, QT interval (representing action potential duration) decreased, and T wave amplitude and slopes of the T wave ascending and descending limbs progressively increased. The changes in conduction velocity were different than those of the electrogram properties, slightly increasing during the first 3 to 5 days and gradually declining toward day 8 in culture.Conclusion: Establishment of uniform activation patterns in spontaneously firing or driven myocytes in monolayer cultures is accompanied by organization of activation and repolarization whose evolution appears in concert with that of a mature connexin43 staining pattern. The experimental techniques developed in this study provide useful tools to investigate the complex relations among gap junctions, conduction velocity, and propagation patterns, as well as a means to learn how gap junctional remodeling under pathophysiologic conditions predisposes the myocardium to arrhythmias.