Liquid chromatography assay for 5-aminolevulinic acid: Application to in vitro assessment of skin penetration via Dermaportation
Liquid chromatography assay for 5-aminolevulinic acid: Application to in vitro assessment of skin penetration via Dermaportation
复制标题
DOI：
10.1016/j.jchromb.2006.12.040
复制
发表时间：
2007-06-01
影响因子：
3
通讯作者：
Benson, Heather A. E.
中科院分区：
文献类型：
--
作者：
Namjoshi, Sarika;Caccetta, Rima;Benson, Heather A. E.
本研究的目的是建立反相高效液相色谱法（HPLC）定量测定5-氨基乙酰丙酸（ALA）。本实验应用于研究ALA的皮肤渗透和一种新的皮肤渗透增强技术的影响。利用Phenomenex Jupiter C-18色谱柱进行荧光衍生化分离。该方法线性（r(2) > 0.99），最小定量限为400 ng/mL。日内、间波动在线性区间的下端分别为1.6、0.9%，上端分别为1.5、1.9%。与其他荧光衍生化方法相比，高效液相色谱分析和荧光衍生化方法具有灵敏、简单、快速、准确和重复性好等优点。初步皮肤渗透研究的结果表明，只有当与dermaporation一起作为皮肤渗透增强装置时，ALA才会有大量的皮肤渗透。(c) 2007 Elsevier B.V.版权所有
The purpose of the present study was to develop a reverse-phase high performance liquid chromatographic (HPLC) assay for quantifying 5-aminolevulinic acid (ALA). The assay was applied to study the skin permeation of ALA and the influence of a novel skin penetration enhancement technology. Separation was achieved utilizing a Phenomenex Jupiter C-18 column following fluorescence derivatization with fluorescamine. The assay was linear (r(2) > 0.99) with a minimum limit of quantitation of 400 ng/mL. The inter- and intraday variation was 1.6 and 0.9% at the lower end of the linear range and 1.5 and 1.9% at the upper end, respectively. The HPLC assay and fluorescence derivatization procedure is sensitive, simple, rapid, accurate and reproducible and offers advantages with regard to stability of ALA in comparison to other fluorescence derivatization methods. Results from the preliminary skin permeation study demonstrated substantial skin penetration of ALA only when applied with Dermaportation as a skin penetration enhancement device. (c) 2007 Elsevier B.V. All rights reserved.