Colorimetric silver detection of DNA microarrays
Colorimetric silver detection of DNA microarrays
复制标题
DOI：
10.1006/abio.2001.5176
复制
发表时间：
2001-08-01
影响因子：
2.9
通讯作者：
Remacle, J
中科院分区：
文献类型：
--
作者：
Alexandre, I;Hamels, S;Remacle, J
微阵列的发展通过微型化和多参数特征彻底改变了基因表达分析和分子诊断。影响微阵列上靶杂交检测效率的关键因素是捕获探针的设计、它们与支持物的连接方式以及检测方法的灵敏度。微阵列目前使用复杂的共聚焦激光扫描仪在荧光中检测。在这项工作中，我们提出了一种新的比色检测方法，旨在使微阵列的使用在研究和临床环境中的一个强大的程序和低成本的工具。用这种方法产生的信号是由于银沉淀到与链霉亲和素结合的纳米金颗粒上，后者用于检测生物素化的DNA。将该比色法与Cy-3荧光法进行了比较。这两种方法的检测限是相当的，相当于1阿莫尔的生物素标记的DNA连接在一个阵列上。扫描和数据分析的阵列获得了基于比色的工作站。(C)北京：科学出版社.
Development of microarrays has revolutionized gene expression analysis and molecular diagnosis through miniaturization and the multiparametric features. Critical factors affecting detection efficiency of targets hybridization on microarray are the design of capture probes, the way they are attached to the support, and the sensitivity of the detection method. Microarrays are currently detected in fluorescence using a sophisticated confocal laser-based scanner. In this work, we present a new colorimetric detection method which is intended to make the use of microarray a powerful procedure and a low-cost tool in research and clinical settings. The signal generated with this method results from the precipitation of silver onto nanogold particles bound to streptavidin, the latter being used for detecting biotinylated DNA. This colorimetric method has been compared to the Cy-3 fluorescence method. The detection limit of both methods was equivalent and corresponds to 1 amol of biotinylated DNA attached on an array. Scanning and data analysis of the array were obtained with a colorimetric-based workstation. (C) 2001 Academic Press.