Vestibular and visual climbing fiber signals evoked in the uvula-nodulus of the rabbit cerebellum by natural stimulation
Vestibular and visual climbing fiber signals evoked in the uvula-nodulus of the rabbit cerebellum by natural stimulation
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DOI：
10.1152/jn.1995.74.6.2573
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发表时间：
1995-12-01
影响因子：
2.5
通讯作者：
Shojaku, H
中科院分区：
文献类型：
--
作者：
Barmack, NH;Shojaku, H
1.小脑悬雍垂-结节接受初级和次级前庭传入纤维以及前庭相关攀爬纤维的前庭投射。它还接收来自攀爬纤维通路的视觉相关信息。在这个实验中，我们研究了这些信息是如何映射到悬雍垂结节。研究结果：1.在α-氯醛糖麻醉的家兔中，研究前庭刺激和视动刺激诱发的攀爬纤维反应（CFRs）、简单棘波反应和杂乱纤维末梢反应的特异性、动力学和地形分布。前庭诱发的CFR被发现在腹侧悬雍垂和结节。这些反应引起静态滚转倾斜的兔子在纵轴和正弦振荡的纵轴。耳的静态反应归因于重力的线性加速度对椭圆囊耳石的刺激。缺乏静态成分的CFR归因于半规管的激活。使用“零技术”，我们表明，运河敏感的CFRs引起的刺激前或后半规管。在归类为管相关的CFR中，96%可归因于垂直半规管的刺激。CFRs的增加与我们记录的一半浦肯野细胞中简单棘波反应的减少相关。这些攀爬纤维诱导的简单尖峰的停顿发生在自发的攀爬纤维放电以及前庭刺激诱发的攀爬纤维放电过程中。这种停顿的持续时间与CFR发生前简单峰电位的自发水平成反比。在另一半记录的浦肯野细胞群体中，前庭驱动的CFR没有改变简单的棘波反应。前庭和视觉介导的CFRs的悬雍垂结节的表面上的地形表示。由同侧耳石输入驱动的CFRs分布在悬雍垂结节的整个中外侧表面。由同侧后半规管驱动的CFRs呈矢状带状分布，宽约1.5 mm，从结节中线向外延伸。完全由同侧眼的水平、后-->前视动刺激驱动的CFR分布在距离中线0.5-1.0 mm处的矢状带中，宽约0.5 mm，并局限于腹侧结节。由同侧前半规管驱动的CFRs呈矢状带状，宽约1.0 mm，自中线延伸1.0-2.0 mm。矢状面，地形排列的攀爬纤维条有效地映射基于前庭和视动信息的可能的姿势反应的中外侧梯度。
1. The cerebellar uvula-nodulus receives vestibular projections from primary and secondary vestibular afferents as well as vestibularly related climbing fibers. It also receives visually related information from climbing fiber pathways. In this experiment we investigated how this information is mapped onto the uvula-nodulus. We studied the specificity, dynamics, and topographic distribution of climbing fiber responses (CFRs), simple spike responses, and messy fiber terminal responses evoked by vestibular and optokinetic stimulation in rabbits anesthetized with alpha-chloralose.2. Vestibularly evoked CFRs were found in the ventral uvula and nodulus. These responses were evoked during static roll tilt of the rabbit about a longitudinal axis and by sinusoidal oscillation about the longitudinal axis. Purely static responses were attributed to stimulation of the utricular otolith by the linear acceleration of gravity. CFRs that lacked a static component were attributed to activation of the semicircular canals.3. Using a ''null technique'' we showed that the canal-sensitive CFRs were caused by stimulation of the anterior or posterior semicircular canals. Of the CFRs classified as canal related, 96% could be attributed to stimulation of the vertical semicircular canals.4. Increases in CFRs were correlated with decreases in simple spike responses in half the Purkinje cells from which we recorded. These climbing-fiber-induced pauses in simple spikes occurred during spontaneous climbing fiber discharge as well as during climbing fiber discharge evoked by vestibular stimulation. The duration of this pause was inversely proportional to the spontaneous level of simple spikes before the occurrence of a CFR. In the other half of the recorded population of Purkinje cells, vestibularly driven CFRs did not alter the simple spike responses.5. Vestibularly and visually mediated CFRs were topographically represented on the surface of the uvula-nodulus. CFRs driven by ipsilateral otolithic inputs were distributed over the entire mediolateral surface of the uvula-nodulus. CFRs driven by the ipsilateral posterior semicircular canal were distributed in a sagittal strip similar to 1.5 mm wide, extending laterally from the midline of the nodulus. CFRs driven exclusively by horizontal, posterior --> anterior optokinetic stimulation of the ipsilateral eye were distributed in a sagittal strip similar to 0.5 mm wide located 0.5-1.0 mm from the midline and restricted to the ventral nodulus. CFRs driven by the ipsilateral anterior semicircular canal were found in a sagittal strip similar to 1.0 mm wide extending 1.0-2.0 mm from the midline.6. The sagittal, topographically arrayed climbing fiber strips effectively map a mediolateral gradient of possible postural responses based on vestibular and optokinetic information.