Specific chromosomal abnormalities in malignant human gliomas.
Specific chromosomal abnormalities in malignant human gliomas.
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发表时间：
1988-01
期刊：
影响因子：
11.2
通讯作者：
S. Bigner;J. Mark;P. Burger;M. Mahaley;D. Bullard;L. Muhlbaier;D. Bigner
中科院分区：
文献类型：
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作者：
S. Bigner;J. Mark;P. Burger;M. Mahaley;D. Bullard;L. Muhlbaier;D. Bigner
对54例恶性胶质瘤(间变性星形细胞瘤5例，多形性胶质母细胞瘤43例，胶质肉瘤3例，巨细胞胶质母细胞瘤2例，间变性混合性胶质瘤1例)的核型分析表明，12例肿瘤具有正常的干系或仅缺少一条性染色体。核型异常的42个肿瘤中，38个肿瘤可完全分析。其中6例具有近三倍体或近四倍体干系，32例具有近二倍体干系。在近二倍体组中，有统计学意义的数字偏差是7号染色体获得(32例中26例，P<0.001)，10号染色体缺失(32例中19例，P<0.001)。在32个近二倍体肿瘤中，有18个肿瘤出现双分钟。通过比较每条染色体臂上断裂点的发生率与基于染色体臂长度的期望值，对结构异常的分布进行统计分析。分析表明，9p和19q的结构异常具有统计学意义(P<0.005.0 1和P=0.0 2)。尽管1号染色体、6p号染色体、11号染色体着丝粒区域、13q号和15q号染色体也经常发生结构异常，但这些断裂的发生率没有达到统计学意义。这种近二倍体胶质瘤中特定染色体异常的证明为研究这些肿瘤中可能发生定量或定性改变的基因提供了基础。
Karyotypic analysis of 54 malignant human gliomas (5 anaplastic astrocytomas, 43 glioblastoma multiformes, 3 gliosarcomas, 2 giant cell glioblastomas, 1 anaplastic mixed glioma) has demonstrated that 12 tumors contained normal stemlines or only lacked one sex chromosome. The 42 tumors with abnormal karyotypes included 38 tumors which could be completely analyzed. Six of these 38 cases had near-triploid or near-tetraploid stemlines and 32 had near-diploid stemlines. Statistically significant numerical deviations in the near-diploid group were gains of chromosome 7 (26 of 32; P less than 0.001), and losses of chromosome 10 (19 of 32; P less than 0.001). Double minutes occurred in 18 of 32 near diploid tumors. The distribution of structural abnormalities was analyzed statistically by comparing the incidence of breakpoint in each chromosomal arm to the expected value based on chromosomal arm length. This analysis demonstrated that structural abnormalities of 9p and 19q were significant statistically (P less than 0.005 and P = 0.02, respectively). Although chromosome 1, 6p, the centromeric region of chromosome 11, 13q, and 15q were also frequently involved in structural abnormalities, the incidence of these breaks did not reach statistical significance. This demonstration of specific chromosomal abnormalities in near-diploid gliomas provides the basis for the investigation of genes which may be quantitatively or qualitatively altered in these neoplasms.