Detection of canine parvovirus type 2c by a commercially available in-house rapid test
Detection of canine parvovirus type 2c by a commercially available in-house rapid test
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DOI：
10.1016/j.tvjl.2009.04.006
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发表时间：
2010-06-01
影响因子：
2.2
通讯作者：
Buonavoglia, Canio
中科院分区：
文献类型：
--
作者：
Decaro, Nicola;Desario, Costantina;Buonavoglia, Canio
犬细小病毒(CPV)感染的诊断通常是通过快速免疫层析法进行的，但这些试验检测所有CPV变种的能力，包括最近发现的CPV-2c，仍然存在争议。为了确定这些检测方法是否检测到不同的CPV变种，使用商业上可用的内部检测方法对201份CPV PCR阳性的粪便样本或直肠拭子进行了检测。样本(CPV-2a，n=51；CPV-2b，n=50；CPV-2c，n=100)的DNA载量为10(5)个DNA拷贝/mg粪便。CPV 2a、2b和2c型室内检测的阳性百分率分别为80.4%、78.0%和77.0%，证实了该检测方法对新的CPV-2c变异株的检测能力。然而，考虑到以前观察到的内部检测的敏感性限度，内部检测试剂盒的阴性结果应该通过基于聚合酶链式反应的方法来确认。(C)2009爱思唯尔有限公司。保留所有权利。
Diagnosis of canine parvovirus (CPV) infection is usually carried out by means of rapid immunochromatographic assays, but the ability of these tests to detect all CPV variants, including the recently identified CPV-2c, is still debated. To determine if the assays detect the different CPV variants, 201 CPV PCR-positive faecal samples or rectal swabs were tested using a commercially available in-house test. Specimens (CPV-2a, n = 51; CPV-2b, n = 50; CPV-2c, n = 100), containing CPV DNA loads >10(5) DNA copies/mg faeces, as determined by real-time PCR, were selected from previous studies. The percentage of positive in-house tests was 80.4%, 78.0% and 77.0% for CPV types 2a, 2b and 2c, respectively, confirming the ability of the test to detect the new variant CPV-2c. However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods. (C) 2009 Elsevier Ltd. All rights reserved.