PLASMID SPECIFYING TOTAL DEGRADATION OF 3-CHLOROBENZOATE BY A MODIFIED ORTHO-PATHWAY
PLASMID SPECIFYING TOTAL DEGRADATION OF 3-CHLOROBENZOATE BY A MODIFIED ORTHO-PATHWAY
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DOI：
10.1128/jb.146.2.639-646.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
CHAKRABARTY, AM
中科院分区：
文献类型：
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作者：
CHATTERJEE, DK;KELLOGG, ST;CHAKRABARTY, AM
一个质粒，称为pAC 25，指定的3-氯苯甲酸在恶臭假单胞菌菌株的生物降解的特点。在含有质粒的细胞与3-氯苯甲酸盐的生长过程中，3-氯儿茶酚和β-邻苯二酚积累。氯粘康酸作为中间体，并且以无机氯化物的形式释放> 80%的Cl。该质粒的平均分子量为68 × 104。106道尔顿，并传播给一些假单胞菌属。例如铜绿假单胞菌、恶臭假单胞菌菌株PpG 1和恶臭假单胞菌菌株PRS 1。将pAC 25转移到恶臭假单胞菌菌株PRS 1的各种儿茶酚阴性突变体中表明，染色体编码的焦儿茶酶不被质粒特异性焦儿茶酶补充，该酶似乎对氯化儿茶酚具有特异性。与苯甲酸盐相反，苯甲酸盐通过邻位途径通过β-当质粒以酮己二酸酯作为中间体时，质粒通过马来酰乙酸酯作为中间体指定氯儿茶酚的邻位裂解。
A plasmid, termed pAC25, specifying biodegradation of 3-chlorobenzoate in a strain of Pseudomonas putida was characterized. During growth of the plasmid-harboring cells with 3-chlorobenzoate, there was an accumulation of 3-chlorocatechol and .beta.-chloromuconic acid as intermediates and release of > 80% of the Cl in the form of inorganic chloride. The plasmid had a mean molecular mass of 68 .times. 106 daltons and was transmissible to a number of Pseudomonas spp. such as P. aeruginosa, P. putida strain PpG1 and P. putida strain PRS1. Transfer of pAC25 to various catechol-negative mutants of P. putida strain PRS1 showed that the chromosomally coded pyrocatechase was not complemented by the plasmid-specified pyrocatechase, which appeared to be specific for the chlorinated catechols. In contrast to benzoate, which was metabolized by the ortho pathway through .beta.-ketoadipate as an intermediate, the plasmid specified ortho cleavage of the chlorocatechols through maleylacetate as an intermediate.