Holographic immunoassays: direct detection of antibodies binding to colloidal spheres
Holographic immunoassays: direct detection of antibodies binding to colloidal spheres
复制标题
全息免疫分析：直接检测与胶体球结合的抗体
DOI：
10.1039/d0sm01351j
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发表时间：
2020
期刊：
影响因子：
3.4
通讯作者：
Grier, David G.
中科院分区：
文献类型：
--
作者：
Snyder, Kaitlynn;Quddus, Rushna;Hollingsworth, Andrew D.;Kirshenbaum, Kent;Grier, David G.
通过全息颗粒表征报告的探针珠的大小取决于被结合的靶分子覆盖的表面积的比例，因此可以用作分子结合的测定。我们通过测量抗体免疫球蛋白G（IgG）和免疫球蛋白M（IgM）与包被有蛋白A的微米直径胶体珠的不可逆结合动力学来验证该技术。这些测量产生抗体的结合率，并且可以被反转以获得溶液中抗体的浓度。因此，全息分子结合测定可用于进行快速定量免疫测定，其是对常规血清学测试的补充。
The size of a probe bead reported by holographic particle characterization depends on the proportion of the surface area covered by bound target molecules and so can be used as an assay for molecular binding. We validate this technique by measuring the kinetics of irreversible binding for the antibodies immunoglobulin G (IgG) and immunoglobulin M (IgM) as they attach to micrometer-diameter colloidal beads coated with protein A. These measurements yield the antibodies’ binding rates and can be inverted to obtain the concentration of antibodies in solution. Holographic molecular binding assays therefore can be used to perform fast quantitative immunoassays that are complementary to conventional serological tests.