A versatile ratiometric nanosensing approach for sensitive and accurate detection of Hg2+ and biological thiols based on new fluorescent carbon quantum dots
A versatile ratiometric nanosensing approach for sensitive and accurate detection of Hg2+ and biological thiols based on new fluorescent carbon quantum dots
复制标题
一种基于新型荧光碳量子点的多功能比率纳米传感方法，可灵敏准确地检测 Hg2 和生物硫醇
DOI：
10.1007/s00216-017-0183-3
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发表时间：
2017
影响因子：
4.3
通讯作者：
Jinmao You
中科院分区：
文献类型：
--
作者：
Huili Fu;Zhongyin Ji;Xuejie Chen;Anwei Cheng;Shucheng Liu;Peiwei Gong;Guoliang Li;Guang Chen;Zhiwei Sun;Xianen Zhao;Feng Cheng;Jinmao You
本文首次报道了一种以海藻酸钠为碳源，组氨酸为氮源和功能单体，采用一锅法水热合成高荧光碳量子点的方法。所制备的CQD给出了32%的高量子产率。通过使用新的CQDs和罗丹明B（RhB），我们展示了一个简单，方便，灵敏，准确的比率传感器检测汞2+和生物硫醇。由于Hg 2+与CQDs表面官能团之间的强静电相互作用，Hg 2+可以选择性地强烈抑制CQDs的光致发光。当谷胱甘肽（GSH）被引入到“关闭”CQDs-RhB-Hg 2+传感系统中时，由于巯基与Hg 2+之间的更强亲和力，CQDs的荧光可以迅速恢复，而RhB的荧光在该传感过程中保持恒定。基于上述原理，可以容易地实现用于检测Hg 2+和GSH的比率计量策略，并且对Hg 2+和GSH分别给出令人满意的检测限（LOD）为30和20 nM。双发射荧光CQDs-RhB传感器不需要复杂的分子设计和双发射荧光团的合成。同时，研究了该方法分析水样、食品样品和生物样品（小鼠氧化应激研究的血浆）的可行性。实验结果表明，所研制的比例型纳米传感器具有简单、样品消耗少、快速、成本低、灵敏度高、选择性好等优点，可用于汞离子和生物硫醇的检测，为环境、食品和生物分析提供了新的方法。Hg 2+和生物硫醇的比率纳米传感方法检测。
Herein, we first reported a facile synthesis method for fabrication of highly photoluminescent carbon quantum dots (CQDs) using sodium alginate as the carbon source and histidine as both the nitrogen source and functional monomer by one-pot hydrothermal synthesis. The as-prepared CQDs gave a high quantum yield of 32%. By employing the new CQDs and rhodamine B (RhB), we demonstrated a simple, facile, sensitive, and accurate ratiometric sensor for detection of Hg2+and biological thiols. The photoluminescence of CQDs in the ratiometric sensor can be selectively and intensively suppressed by Hg2+due to strong electrostatic interaction between the surface functional groups of the CQDs and Hg2+. When glutathione (GSH) was introduced into the "Turn Off" CQDs-RhB-Hg2+sensing system, the fluorescence of the CQDs can be recovered rapidly due to the stronger affinity between thiol and Hg2+, while the fluorescence of the RhB remained constant in this sensing process. Based on the above principle, the ratiometric strategy for detecting Hg2+and GSH can be achieved readily, and gives satisfactory limit of detections (LODs) of 30 and 20 nM for Hg2+and GSH, respectively. The dual-emission fluorescent CQDs-RhB sensor does not need the complicated molecular design and the synthesis of dual-emission fluorophores. Meanwhile, the feasibility of the proposed method for analysis of water samples, food samples, and biological samples (plasma from mice oxidative stress study) was investigated. The developed ratiometric nanosensor is proven to be facile, with less sample consumption, rapid, lost cost, highly sensitive, and very selective for Hg2+and biological thiol detection, which offers a new approach for environmental, food, and biological analysis. Graphical abstract Ratiometric nanosensing approach detection of Hg2+and biological thiols.