Immunofluorescence characterization of key extracellular matrix proteins in murine bone marrow in situ
Immunofluorescence characterization of key extracellular matrix proteins in murine bone marrow in situ
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DOI：
10.1177/002215549804600311
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发表时间：
1998-03-01
影响因子：
3.2
通讯作者：
Becker, PS
中科院分区：
文献类型：
--
作者：
Nilsson, SK;Debatis, ME;Becker, PS
造血干细胞归巢到骨髓的机制涉及分子相互作用，介导这些细胞与骨髓微环境（包括细胞外基质）的识别和相互作用。在选择性结合上，这种环境与可溶性细胞因子结合，调节干细胞的增殖和分化。利用免疫荧光标记，我们分析了小鼠股骨髓切片中突出的细胞外基质蛋白纤维连接蛋白、I型、III型和IV型胶原蛋白和层粘连蛋白的位置。I型、IV型胶原蛋白和纤维连接蛋白定位于内膜，这是股骨微环境的区域，归巢干细胞对该区域具有高度亲和力。结果进一步表明，IV型胶原和层粘连蛋白与骨髓血管（包括小动脉、静脉和鼻窦）具有很强的空间相关性。纤维连接蛋白分布在骨髓中心区域，除III型胶原蛋白外，所有分析蛋白均存在于骨中，尽管水平不同。骨膜中也有纤维连接蛋白、III型和IV型胶原蛋白和层粘连蛋白。特定细胞外基质蛋白的独特位置支持了它们可能在移植细胞的归巢中发挥重要机制作用的观点。
The mechanism of hemopoietic stem cell homing to the bone marrow involves molecular interactions that mediate the recognition and interaction of these cells with the marrow microenvironment, including the extracellular matrix. On selective binding, this environment, in combination with soluble cytokines, regulates stem cell proliferation and differentiation. Using immunofluorescence labeling, we analyzed the location of the prominent extracellular matrix proteins fibronectin, collagen Types I, III, and IV, and laminin in sections of murine femoral bone marrow. Collagen Types I, IV, and fibronectin were localized to the endosteum, the region of the femoral microenvironment for which homing stem cells have a high affinity. The results further demonstrated a strong spatial association of collagen Type IV and laminin with the bone marrow vessels, including arterioles, veins, and sinuses. Fibronectin was distributed throughout the central marrow region, and all the proteins analyzed except collagen Type III were present in the bone, although at different levels. Fibronectin, collagen Types III and IV, and laminin were also present in the periosteum. The distinct locations of particular extracellular matrix proteins support the notion that they may play an important mechanistic role in the homing of engrafting cells.