pirA- and pirB-like gene identification in Micrococcus luteus strains in Mexico
pirA- and pirB-like gene identification in Micrococcus luteus strains in Mexico
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DOI：
10.1111/jfd.12874
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发表时间：
2018-11-01
影响因子：
2.5
通讯作者：
Vibanco-Perez, Norberto
中科院分区：
文献类型：
--
作者：
de Jesus Duran-Avelar, Ma.;Vazquez-Reyes, Alejandro;Vibanco-Perez, Norberto
急性肝胰腺坏死病（AHPND）于2009年首次在中国报告，随后于2013年在墨西哥报告。AHPND由副溶血性弧菌引起，影响斑节对虾和凡纳滨对虾养殖。这种细菌在69至70 Kb的质粒中含有pirA和pirB样基因，这些基因编码产生疾病的毒素。本研究的目的是确定是否pirA和pirB样基因存在于不同的弧菌属的第一例AHPND记录在墨西哥之前。2006年从纳亚里特的虾养殖场选择了两种细菌分离株，并通过巢式PCR进行分析，以确定pirA和pirB样基因的存在。选择的两个分离株确实显示了这些基因的存在，这些发现通过测序得到了证实。这两种菌株都与细菌物种藤黄微球菌相匹配。结果揭示了两个重要的情况：（a）pirA和pirB样基因存在于一种以前未报道的细菌物种（藤黄微球菌）中;（B）在中国首次报告AHPND暴发之前，墨西哥就存在pirA和pirB样细菌基因。
Acute hepatopancreatic necrosis disease (AHPND) was first reported in China in 2009 and afterwards in Mexico in 2013. AHPND is caused by Vibrio parahaemolyticus and affects Penaeus monodon and Litopenaeus vannamei shrimp cultures. The bacterium contains the pirA- and pirB-like genes in 69- to 70-Kb plasmids, which encode the toxins that produce the disease. The aim of this study was to determine whether pirA- and pirB-like genes existed in bacterial genera distinct from Vibrio before the first cases of AHPND were documented in Mexico. Two bacterial isolates were selected from shrimp farms in Nayarit in 2006 and analysed by nested-PCR to determine the presence of pirA- and pirB-like genes. The two isolates chosen did indeed show the presence of these genes, and those findings were confirmed by sequencing. Both strains matched to the bacterial species Micrococcus luteus. Results revealed two important situations: (a) the pirA- and pirB-like genes were present in a bacterial species that has not been reported previously (Micrococcus luteus); and (b) pirA- and pirB-like bacterial genes were present in Mexico before the first AHPND outbreak was reported in China.