Mixotrophic growth regime of novel strain Scenedesmus sp. DDVG I in municipal wastewater for concomitant bioremediation and valorization of biomass
Mixotrophic growth regime of novel strain Scenedesmus sp. DDVG I in municipal wastewater for concomitant bioremediation and valorization of biomass
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DOI：
10.1016/j.jclepro.2022.132834
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发表时间：
2022-09-10
影响因子：
11.1
通讯作者：
Hu, Bo
中科院分区：
文献类型：
--
作者：
Devi, Nongmaithem Debeni;Sun, Xiao;Hu, Bo
用于微藻商业化培养的具有成本效益的营养物的可用性是主要挑战之一。本研究调查了从美国明尼苏达州当地污水处理厂收集的初级城市污水（PMWW）作为淡水微藻生长介质的替代品用于高价值生物能源原料生产的可行性。在250 mL锥形瓶中，在PMWW中以异养和兼养模式培养新菌株Scenedesmus sp. DDVG I。将优化的培养模式进一步放大到3L鼓泡生物反应器中。该研究证实了废水作为一个潜在的生长介质，而栅藻物种DDVG I显示出上级的生物量生产力（0.069 g/L d），脂质产量（22.5%），FAME含量（22.04%，细胞干重）超过10天的混合营养模式。生物量也显示出较高的必需氨基酸含量（159.8 mg/g DCW）。栅藻对COD和TN的生物修复效率分别为75.6%和99.8%。同时，氨氮（NH3-N）和总磷（TP）的去除率高达100%。生物柴油表征所需的脂肪酸谱和不同参数的分析揭示了栅藻衍生油的高品质性质。总体而言，该研究得出结论，有效的城市污水生物修复栅藻属物种。DDVG I与有价值的资源，从生物质的回收。
Availability of cost-effective nutrients for the commercial cultivation of microalgae is one of the major challenges. The present study investigated the feasibility of primary municipal wastewater (PMWW) collected from a local wastewater treatment plant in Minnesota, USA as an alternative to fresh-water microalgae growth media towards high-value bioenergy feedstock production. The novel strain Scenedesmus sp. DDVG I was cultivated in the PMWW under heterotrophic and mixotrophic modes in 250 mL Erlenmeyer flasks. The optimized cultivation mode was further scaled up to the 3-L bubbled bioreactor. The study confirmed the wastewater as a potential growth medium while Scenedesmus sp. DDVG I showed superior biomass productivity (0.069 g/L d), lipid yield (22.5%), and FAME content (22.04% in dry cell weight) over 10 days in the mixotrophic mode. The biomass also showed high essential amino acid content (159.8 mg/g DCW). The corresponding values for bioremediation efficiencies of chemical oxygen demand (COD) and total nitrogen (TN) by Scenedesmus sp. were 75.6% and 99.8% respectively. Meanwhile, ammonia nitrogen (NH3-N) and total phosphorus (TP) were removed at up to 100% removal efficiencies. Analyses of fatty acid profile and different parameters that were required for biodiesel characterization revealed the high-quality nature of the Scenedesmus-derived oil. Overall, the study concluded effective bioremediation of municipal wastewater by Scenedesmus sp. DDVG I with the recovery of valuable resources from the biomass.