Immunocytochemical demonstration of extracellular matrix proteins in isolated osteocytes
Immunocytochemical demonstration of extracellular matrix proteins in isolated osteocytes
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DOI：
10.1007/bf02473312
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发表时间：
1996-11-01
影响因子：
2.3
通讯作者：
Nijweide, PJ
中科院分区：
文献类型：
--
作者：
Aarden, EM;Wassenaar, AMM;Nijweide, PJ
分离骨细胞的培养可以提供一个合适的系统来研究骨细胞的功能，因为在培养中分离的骨细胞的行为非常像在体内的骨细胞。本文研究了骨细胞通过生成基质蛋白改变其周围细胞外基质的能力。我们用免疫细胞化学方法测定了分离的鸡骨细胞、成骨细胞和骨膜成纤维细胞培养物中I型胶原、纤维连接蛋白、骨钙素、骨桥蛋白和骨连接蛋白的存在。在成骨细胞和骨膜成纤维细胞培养中，形成了大的I型胶原蛋白和纤维连接蛋白的细胞外网络；但在骨细胞群体中，很少发现胶原蛋白或纤维连接蛋白的细胞外线。另一方面，高尔基体中骨钙素、骨连接素和骨桥蛋白阳性的细胞百分比在骨细胞群中最高。这些结果表明，骨细胞有能力通过产生基质蛋白来改变其周围细胞外基质的组成。我们认为这种特性对于细胞周围骨基质钙化的调节是重要的。更重要的是，由于骨细胞作为机械传感器细胞的作用依赖于它们与基质蛋白的相互作用，因此对周围基质的适应提供了一种调节它们对机械负荷反应的方法。
Cultures of isolated osteocytes may offer an appropriate system to study osteocyte function, since isolated osteocytes in culture behave very much like osteocytes in vivo. In this paper we studied the capacity of osteocytes to change their surrounding extracellular matrix by production of matrix proteins. With an immunocytochemical method we determined the presence of collagen type I, fibronectin, osteocalcin, osteopontin and osteonectin in cultures of isolated chicken osteocytes, osteoblasts and periosteal fibroblasts. In osteoblast and periosteal fibroblast cultures, large extracellular networks of collagen type I and fibronectin were formed: but in osteocyte populations, extracellular threads of collagen or fibronectin were only rarely found. The percentage of cells positive for osteocalcin, osteonectin and osteopontin in the Golgi apparatus, on the other hand, was highest in the osteocyte population. These results show that osteocytes have the ability to alter the composition of their surrounding extracellular matrix by producing matrix proteins. We suggest this property is of importance for the regulation of the calcification of the bone matrix immediately surrounding the cells. More importantly, as osteocytes depend for their role as mechanosensor cells on their interaction with matrix proteins, the adaptation of the surrounding matrix offers a way to regulate their response to mechanical loading.