RNA-binding proteins.
RNA-binding proteins.
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DOI：
10.1895/wormbook.1.79.1
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发表时间：
2006-04
期刊：
影响因子：
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通讯作者：
Min‐ho Lee;T. Schedl
中科院分区：
文献类型：
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作者：
Min‐ho Lee;T. Schedl
秀丽隐杆线虫的基因组编码了许多RNA结合蛋白（rbp），这些RNA结合蛋白在发育过程中具有不同的功能，表明RNA代谢的转录后控制具有广泛的层次。许多秀丽隐杆线虫的rbp已通过正向或反向遗传学鉴定。它们倾向于显示组织特异性突变表型，这强调了它们的功能重要性。此外，已经从分子上鉴定了几种结合mrna 3'非翻译区调控序列的rbp。大多数秀丽隐杆线虫的rbp在整个进化过程中都是保守的，这表明它们在秀丽隐杆线虫中的研究可能会发现新的保守的生物学功能。在这篇综述中，我们主要讨论与生殖系、早期胚胎或体细胞组织中具有良好特征的突变表型相关的rbp。我们还讨论了RBP的RNA靶点鉴定，这是了解RBP如何控制秀丽隐杆线虫发育的重要的第一步。大多数rbp可能调控多个RNA靶点。一旦确定了多个RNA靶标，就可以确定区分靶标RNA和非靶标RNA的具体特征以及每个RBP控制的RNA代谢类型。此外，我们可以确定RBP是通过相同的机制调节所有靶点，还是通过不同的机制调节不同的靶点。这些研究将为rbp如何协调控制其RNA靶标，从而以协调一致的方式影响发育提供见解。
The C. elegans genome encodes many RNA-binding proteins (RBPs) with diverse functions in development, indicative of extensive layers of post-transcriptional control of RNA metabolism. A number of C. elegans RBPs have been identified by forward or reverse genetics. They tend to display tissue-specific mutant phenotypes, which underscore their functional importance. In addition, several RBPs that bind regulatory sequences in the 3'untranslated regions of mRNAs have been identified molecularly. Most C. elegans RBPs are conserved throughout evolution, suggesting that their study in C. elegans may uncover new conserved biological functions. In this review, we primarily discuss RBPs that are associated with well-characterized mutant phenotypes in the germ line, the early embryo, or in somatic tissues. We also discuss the identification of RNA targets of RBPs, which is an important first step to understand how an RBP controls C. elegans development. It is likely that most RBPs regulate multiple RNA targets. Once multiple RNA targets are identified, specific features that distinguish target from non-target RNAs and the type(s) of RNA metabolism that each RBP controls can be determined. Furthermore, one can determine whether the RBP regulates all targets by the same mechanism or different targets by distinct mechanisms. Such studies will provide insights into how RBPs exert coordinate control of their RNA targets, thereby affecting development in a concerted fashion.