Intestinal water and solute absorption studies: comparison of in situ perfusion with chronic isolated loops in rats.
Intestinal water and solute absorption studies: comparison of in situ perfusion with chronic isolated loops in rats.
复制标题
肠道水和溶质吸收研究：大鼠体内原位灌注与慢性隔离环的比较。
DOI：
10.1023/a:1015848815616
复制
发表时间：
1992
影响因子：
3.7
通讯作者：
Fleisher,D
中科院分区：
文献类型：
--
作者：
Lu,HH;Thomas,JD;Tukker,JJ;Fleisher,D
在麻醉大鼠原位和非麻醉大鼠体内的离体慢性肠袢中，比较了腔内葡萄糖对空肠水转运的影响以及葡萄糖诱导的水吸收对单程灌注溶质摄取的影响。虽然溶质膜渗透性的幅度是一贯较高的慢性环路系统，对水的运输和促进空肠溶质吸收的影响是两个实验系统之间的可比性。在两个实验系统中，葡萄糖诱导的水吸收对亲水性药物对乙酰氨基酚的增强/基线空肠摄取比的影响大于亲脂性药物苯妥英。慢性髓袢有效溶质渗透性与原位溶质膜渗透性相当，这一事实与体内更大的腔内液体混合一致。此外，原位体温影响苯妥英钠的摄取，但不影响对乙酰氨基酚、水或葡萄糖的摄取。这表明，主动和paracellular溶质运输是不compromisedin原位，而亲脂性物质的膜分区和扩散更敏感的实验条件。
The effects of lumenal glucose on jejunal water transport and the influence of glucose-induced water absorption on solute uptake from single-pass perfusions are compared in anesthetized ratsin situand isolated chronic loops in unanesthetized ratsin vivo. While the magnitudes of solute membrane permeabilities are consistently higher in the chronic loop system, the effects on water transport and its promotion of jejunal solute uptake are comparable between the two experimental systems. The effect of glucose-induced water absorption on the enhanced/baseline jejunal uptake ratio of the hydrophilic drug, acetaminophen, is greater than that for the lipophilic drug, phenytoin, in both experimental systems. The fact that chronic loop effective solute permeabilities were equivalent to solute membrane permeabilitiesin situis consistent with greater lumenal fluid mixingin vivo. In addition,in situbody temperature affects the uptake of phenytoin but not acetaminophen, water, or glucose. This suggests that active and paracellular solute transport is not compromisedin situ, while membrane partitioning and diffusion of lipophilic species are more sensitive to experimental conditions.