Hemin-independent control of globin synthesis in Friend erythroleukemia cells induced to differentiate.
Hemin-independent control of globin synthesis in Friend erythroleukemia cells induced to differentiate.
复制标题
诱导分化的 Friend 红白血病细胞中珠蛋白合成的血红素独立控制。
DOI：
10.1073/pnas.79.6.1839
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Friend,C
中科院分区：
文献类型：
--
作者：
Stringer,EA;Friend,C
一种不依赖于氯化血红素的翻译抑制物，当未诱导的Friend白血病(FL)细胞和兔网织红细胞裂解物混合时，它可以防止兔珠蛋白的合成[Cimadevila，J.M.&Hardesy，B.(1975)Biochem]。生物群落。[中英文摘要]Re.Commun.63,931-937]在诱导分化的FL细胞中检测不到。用六亚甲基双乙酰胺或氨基核苷诱导的FL细胞裂解产物与兔网织红细胞混合，不会引起兔珠蛋白合成的抑制。诱导还导致细胞从未诱导的FL细胞获得对抑制物的敏感性。当未诱导和诱导的FL细胞裂解物混合时，观察到总蛋白质合成减少。抑制不是由于未诱导的FL细胞mRNA物种对翻译机制的竞争造成的，因为未诱导的FL细胞裂解产物在微球菌核酸酶处理后仍保持其抑制活性。从与未诱导FL细胞裂解物孵育的兔网织红细胞裂解液中回收的兔珠蛋白mRNA仍能被有效翻译，表明这种抑制不是由于未诱导FL细胞裂解物对其他种类的mRNA的修饰所致。诱导分化后不会切换到氯化血红素依赖的翻译控制。诱导FL细胞裂解物中的氨基酸掺入速率--与未诱导的FL细胞裂解物中的一样--不受系统中省略外源氯化血红素的影响。它的存在并不是防止血红素调节的抑制物激活所必需的。从这些数据可以清楚地看出，FL细胞中蛋白质合成的控制--无论它们是否被诱导--不同于正常红系细胞中氯化血红素对蛋白质合成的调控。
A hemin-independent translational inhibitor that prevents synthesis of rabbit globin when uninduced Friend leukemia (FL) cell and rabbit reticulocyte lysates are mixed [Cimadevilla, J. M. & Hardesty, B. (1975) Biochem. Biophys. Res. Commun. 63, 931-937] cannot be detected in FL cells induced to differentiate. Mixing of lysates of FL cells induced with hexamethylene bisacetamide or aminonucleoside of puromycin and rabbit reticulocytes does not cause inhibition of rabbit globin synthesis. Induction also results in the cells acquiring sensitivity to the inhibitor from uninduced FL cells. A reduction in total protein synthesis is observed when uninduced and induced FL cell lysates are mixed. Inhibition does not result from competition by an excess of uninduced FL cell mRNA species for the translational machinery because uninduced FL cell lysates retain their inhibitory activity after treatment with micrococcal nuclease. Rabbit globin mRNA recovered from rabbit reticulocyte lysates that have been incubated with lysates of uninduced FL cells can still be translated effectively, indicating that inhibition does not result from modification of other species of mRNA by uninduced FL cell lysates. A switch to hemin-dependent translational control does not follow induction of differentiation. The rate of amino acid incorporation in induced FL cell lysates--like that in uninduced FL cell lysates--is unaffected by omission of exogenous hemin from the system. Its presence is not required to prevent activation of heme-regulated inhibitor. From these data, it is clear that the control of protein synthesis in FL cells--whether or not they are induced--is different from that regulated by hemin in normal erythroid cells.