Gradual soil water depletion results in reversible changes of gene expression, protein profiles, ecophysiology, and growth performance in Populus euphratica, a poplar growing in arid regions
Gradual soil water depletion results in reversible changes of gene expression, protein profiles, ecophysiology, and growth performance in Populus euphratica, a poplar growing in arid regions
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DOI：
10.1104/pp.106.088708
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发表时间：
2007-02-01
期刊：
影响因子：
7.4
通讯作者：
Dreyer, Erwin
中科院分区：
文献类型：
--
作者：
Bogeat-Triboulot, Marie-Beatrice;Brosche, Mikael;Dreyer, Erwin
胡杨林（Populus euphratica Oliv.）通过分析基因表达、蛋白质谱和几种植物性能标准来评估植物对土壤水分亏缺的适应性，以了解植物对土壤水分亏缺的适应性。年轻的，植物繁殖的植物起源于干旱，盐碱地网站提交了一个逐渐增加的水赤字4周，在温室中，并允许恢复10天后，充分再灌溉。在地上部和根系的生长，木质部解剖，气体交换和水分状况引起的扰动的时间依赖性的变化和强度进行了记录。在四种胁迫水平和恢复后，在成熟叶和根中（仅基因表达）也记录了大约6，340个基因以及蛋白质和代谢物（色素、可溶性碳水化合物和氧化化合物）的表达谱。干旱连续诱导芽生长停止，气孔关闭，适度增加氧化应激相关化合物，损失的CO2同化，根生长减少。这些影响几乎是完全可逆的，表明驯化是占主导地位的伤害。生理反应被完全可逆的转录变化所掩盖，包括阵列上仅1.5%的基因。蛋白质谱比转录水平显示更大的变化。在阵列上存在表达序列标签的鉴定的蛋白质中，在转录本和蛋白质丰度之间没有发现相关性。对水分亏缺的适应涉及根和芽中不同基因网络的调节。保护和维持不同植物器官功能的这种不同要求可能使植物工程或育种朝着改善耐旱性比以前预期的更复杂。
The responses of Populus euphratica Oliv. plants to soil water deficit were assessed by analyzing gene expression, protein profiles, and several plant performance criteria to understand the acclimation of plants to soil water deficit. Young, vegetatively propagated plants originating from an arid, saline field site were submitted to a gradually increasing water deficit for 4 weeks in a greenhouse and were allowed to recover for 10 d after full reirrigation. Time-dependent changes and intensity of the perturbations induced in shoot and root growth, xylem anatomy, gas exchange, and water status were recorded. The expression profiles of approximately 6,340 genes and of proteins and metabolites (pigments, soluble carbohydrates, and oxidative compounds) were also recorded in mature leaves and in roots (gene expression only) at four stress levels and after recovery. Drought successively induced shoot growth cessation, stomatal closure, moderate increases in oxidative stress-related compounds, loss of CO2 assimilation, and root growth reduction. These effects were almost fully reversible, indicating that acclimation was dominant over injury. The physiological responses were paralleled by fully reversible transcriptional changes, including only 1.5% of the genes on the array. Protein profiles displayed greater changes than transcript levels. Among the identified proteins for which expressed sequence tags were present on the array, no correlation was found between transcript and protein abundance. Acclimation to water deficit involves the regulation of different networks of genes in roots and shoots. Such diverse requirements for protecting and maintaining the function of different plant organs may render plant engineering or breeding toward improved drought tolerance more complex than previously anticipated.