SPECIFIC STIMULATION OF SIMIAN VIRUS-40 LATE TRANSCRIPTION INVITRO BY A CELLULAR FACTOR BINDING THE SIMIAN VIRUS-40 21-BASE-PAIR REPEAT PROMOTER ELEMENT
SPECIFIC STIMULATION OF SIMIAN VIRUS-40 LATE TRANSCRIPTION INVITRO BY A CELLULAR FACTOR BINDING THE SIMIAN VIRUS-40 21-BASE-PAIR REPEAT PROMOTER ELEMENT
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DOI：
10.1073/pnas.84.17.6025
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发表时间：
1987-09-01
影响因子：
11.1
通讯作者：
HANSEN, U
中科院分区：
文献类型：
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作者：
KIM, CH;HEATH, C;HANSEN, U
我们已经确定了未感染的HeLa细胞的细胞转录因子，刺激猿猴病毒40（SV 40）转录的晚期模式，并特异性结合SV 40 21碱基对重复启动子元件。特别地，晚期SV 40转录因子（LSF）刺激SV 40晚期启动子的起始位点L325和L264处的转录，这是在SV 40裂解周期期间DNA复制后利用的主要转录位点。此外，LSF似乎在较低程度上刺激早期启动子的晚期-早期起始位点的转录。LSF特异性地结合到SV 40启动子的21个碱基对重复序列上，形成特异性的蛋白质-DNA复合物，其通过非变性聚丙烯酰胺凝胶的迁移速度比先前鉴定的转录因子Sp1所形成的更快。因此，LSF在其转录和DNA结合特性方面与Sp1不同。这些研究结果表明，在SV 40裂解感染的早期到晚期转录开关LSF的潜在作用。
We have identified a cellular transcription factor from uninfected HeLa cells that stimulates the simian virus 40 (SV40) late mode of transcription and specifically binds the SV40 21-base-pair repeat promoter element. In particular, the late SV40 transcription factor (LSF) stimulates transcription at initiation sites L325 and L264 of the SV40 late promoter, which are the major transcription sites utilized after DNA replication during the SV40 lytic cycle. In addition, LSF appears to stimulate transcription to a lesser extent from the late-early initiation site of the early promoter. LSF binds specifically to the 21-base-pair repeats of the SV40 promoters, forming specific protein-DNA complexes, which migrate more rapidly through nondenaturing polyacrylamide gels than that formed by the previously identified transcription factor Sp1. Thus, LSF is distinguishable from Sp1 in both its transcriptional and DNA-binding properties. These findings suggest a potential role of LSF in the early to late transcriptional switch during a SV40 lytic infection.