Digital holographic microscopy for longitudinal volumetric imaging of growth and treatment response in three-dimensional tumor models.
Digital holographic microscopy for longitudinal volumetric imaging of growth and treatment response in three-dimensional tumor models.
复制标题
数字全息显微镜用于三维肿瘤模型中生长和治疗反应的纵向体积成像。
DOI：
10.1117/1.jbo.19.11.116001
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发表时间：
2014
影响因子：
3.5
通讯作者：
Yelleswarapu,ChandraS
中科院分区：
文献类型：
--
作者：
Li,Yuyu;Petrovic,Ljubica;La,Jeffrey;Celli,JonathanP;Yelleswarapu,ChandraS
我们报告使用数字全息显微镜（DHM）作为一种可行的显微镜方法，定量，无损纵向成像ofin体外三维（3-D）肿瘤模型。根据已建立的方法，我们在细胞外基质床上以重叠几何形状制备了胰腺癌细胞的3-D培养物，并在整个生长期间的多个时间点获得了数字全息图。对全息图进行数字处理，并获得未包裹的相位图像，以量化正常生长下和接受化疗治疗的培养物中随时间推移的结节厚度。通过这种方式，可以快速估算结核总体积，并在此显示生长期间和对治疗的反应中随时间变化的对比。这项工作表明DHM的效用，以量化随着时间的推移在3-D结构的变化，并建议这种方法的进一步发展，在生长过程中的3-D形态变化的延时监测和响应治疗，否则将是不切实际的可视化。
We report the use of digital holographic microscopy (DHM) as a viable microscopy approach for quantitative, nondestructive longitudinal imaging ofin vitrothree-dimensional (3-D) tumor models. Following established methods, we prepared 3-D cultures of pancreatic cancer cells in overlay geometry on extracellular matrix beds and obtained digital holograms at multiple time points throughout the duration of growth. The holograms were digitally processed and the unwrapped phase images were obtained to quantify the nodule thickness over time under normal growth and in cultures subject to chemotherapy treatment. In this manner, total nodule volumes are rapidly estimated and demonstrated here to show contrasting time-dependent changes during growth and in response to treatment. This work suggests the utility of DHM to quantify changes in 3-D structure over time and suggests the further development of this approach for time-lapse monitoring of 3-D morphological changes during growth and in response to treatment that would otherwise be impractical to visualize.