Phosphoenolpyruvate carboxykinase is involved in antiviral immunity against Bombyx mori nucleopolyhedrovirus
Phosphoenolpyruvate carboxykinase is involved in antiviral immunity against Bombyx mori nucleopolyhedrovirus
复制标题
磷酸烯醇丙酮酸羧激酶参与针对家蚕核多角体病毒的抗病毒免疫
DOI：
10.1016/j.dci.2018.11.015
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发表时间：
2019-03-01
影响因子：
2.9
通讯作者：
Xia, Qingyou
中科院分区：
文献类型：
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作者：
Guo, Huizhen;Xu, Guowen;Xia, Qingyou
磷酸烯醇式丙酮酸羧激酶（PEPCK）具有细胞质亚型（PEPCK-C）和线粒体亚型（PEPCK-M）。PEPCK-C在肿瘤发生中起重要作用，但PEPCK-M的功能在很大程度上是未知的。在这项研究中，我们克隆了两个异构体的PEPCK（BmPEPCK-1和BmPEPCK-2;两个PEPCK-M）从鳞翅目模型家蚕。BmPEPCK-1和BmPEPCK-2在家蚕基因组中相邻定位，均含有13个外显子。序列差异主要是第13外显子和3 'UTR。BmPEPCK-1的表达高于BmPEPCK-2，但BmPEPCK-2的过表达不影响BmNPV的增殖。感染BmNPV后，BmPEPCK-2和ATG 6/7/8/13的表达水平下降。BmPEPCK-2的过表达增加了ATG 6/7/8的表达，并显著降低了病毒的荧光和含量，表明BmPEPCK-2通过增加ATGs的表达来抑制BmNPV的增殖。这些结果表明PEPCK-M在抗病毒免疫中具有重要的功能。
Phosphoenolpyruvate carboxykinase (PEPCK) has cytoplasmic isoform (PEPCK-C) and a mitochondrial isoform (PEPCK-M). PEPCK-C plays an important role in gluconeogenesis, but the function of PEPCK-M is largely unknown. In this study, we cloned two isoforms of PEPCK (BmPEPCK-1 and BmPEPCK-2; both of PEPCK-M) from the lepidopteran model Bombyx mori. BmPEPCK-1 and BmPEPCK-2 were adjacently located in the silkworm genome, and both contained 13 exons. The main difference in the sequences was the 13th exon and 3'UTR. The expression of BmPEPCK-1 was higher than that of BmPEPCK-2, the overexpression of which did not affect BmNPV proliferation. The expression levels of BmPEPCK-2 and ATG6/7/8/13 decreased after BmNPV infection. Overexpression of BmPEPCK-2 increased the expression of ATG6/7/8 and significantly decreased viral fluorescence and content, suggesting that BmPEPCK-2 suppressed the multiplication of BmNPV by increasing ATGs expression. These results revealed that PEPCK-M has an important function in antiviral immunity.